172970

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3849336..3850015 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag ID627_RS19000 Protein ID WP_000854680.1
Coordinates 3849674..3850015 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag ID627_RS18995 Protein ID WP_000070396.1
Coordinates 3849336..3849653 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS18950 3844774..3845595 + 822 WP_000197387.1 DUF945 domain-containing protein -
ID627_RS18955 3845812..3846513 + 702 WP_000189411.1 WYL domain-containing protein -
ID627_RS18960 3846554..3846790 + 237 WP_001144031.1 hypothetical protein -
ID627_RS18965 3846790..3847233 + 444 WP_000649865.1 hypothetical protein -
ID627_RS18970 3847256..3847723 + 468 WP_001385283.1 hypothetical protein -
ID627_RS18975 3847800..3848039 + 240 WP_106642549.1 DUF905 domain-containing protein -
ID627_RS18980 3848137..3848595 + 459 WP_000211838.1 antirestriction protein -
ID627_RS18985 3848611..3849087 + 477 WP_000811693.1 RadC family protein -
ID627_RS18990 3849096..3849317 + 222 WP_000691994.1 DUF987 domain-containing protein -
ID627_RS18995 3849336..3849653 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
ID627_RS19000 3849674..3850015 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
ID627_RS19010 3850534..3851607 + 1074 WP_001396517.1 site-specific integrase -
ID627_RS19015 3851617..3852288 + 672 Protein_3746 hypothetical protein -
ID627_RS19020 3852300..3852997 - 698 Protein_3747 IS1 family transposase -
ID627_RS19025 3853151..3854101 - 951 WP_000947155.1 magnesium/cobalt transporter CorA -
ID627_RS26080 3854253..3854309 - 57 WP_211180520.1 protein YsgD -
ID627_RS19030 3854471..3854785 + 315 WP_000951129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3844774..3952820 108046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T172970 WP_000854680.1 NZ_CP061764:3849674-3850015 [Escherichia coli O19:H7]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T172970 NZ_CP080066:2674713-2674820 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT172970 WP_000070396.1 NZ_CP061764:3849336-3849653 [Escherichia coli O19:H7]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT172970 NZ_CP080066:c2674657-2674601 [Escherichia coli]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References