Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2628503..2629141 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ID627_RS12915 Protein ID WP_000813794.1
Coordinates 2628965..2629141 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ID627_RS12910 Protein ID WP_001270286.1
Coordinates 2628503..2628919 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS12890 2623655..2624596 - 942 WP_001251313.1 ABC transporter permease -
ID627_RS12895 2624597..2625610 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
ID627_RS12900 2625628..2626773 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
ID627_RS12905 2627018..2628424 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
ID627_RS12910 2628503..2628919 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ID627_RS12915 2628965..2629141 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ID627_RS12920 2629363..2629593 + 231 WP_000494244.1 YncJ family protein -
ID627_RS12925 2629685..2631646 - 1962 WP_191113565.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ID627_RS12930 2631719..2632255 - 537 WP_001548531.1 DNA-binding transcriptional regulator SutR -
ID627_RS12935 2632308..2633522 + 1215 WP_001324881.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T172968 WP_000813794.1 NZ_CP061764:c2629141-2628965 [Escherichia coli O19:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T172968 NZ_CP080066:1980277-1980380 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT172968 WP_001270286.1 NZ_CP061764:c2628919-2628503 [Escherichia coli O19:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT172968 NZ_CP080066:c1980418-1980147 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References