Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 976691..977345 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ID627_RS04845 Protein ID WP_000244781.1
Coordinates 976938..977345 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ID627_RS04840 Protein ID WP_000354046.1
Coordinates 976691..976957 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS04815 971856..972598 + 743 Protein_954 SDR family oxidoreductase -
ID627_RS04820 972660..974093 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
ID627_RS04825 974138..974449 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ID627_RS04830 974613..975272 + 660 WP_000250274.1 hemolysin III family protein -
ID627_RS04835 975468..976448 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
ID627_RS04840 976691..976957 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ID627_RS04845 976938..977345 + 408 WP_000244781.1 protein YgfX Toxin
ID627_RS04850 977385..977906 - 522 WP_001055874.1 flavodoxin FldB -
ID627_RS04855 978018..978914 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ID627_RS04860 978939..979649 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ID627_RS04865 979655..981388 + 1734 WP_191113491.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T172955 WP_000244781.1 NZ_CP061764:976938-977345 [Escherichia coli O19:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172955 NZ_CP080058:60260-60541 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172955 WP_000354046.1 NZ_CP061764:976691-976957 [Escherichia coli O19:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172955 NZ_CP080058:60019-60270 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References