Detailed information of TA system TA172953

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 747847..748634
• Replicon: chromosome
• Accession: NZ_CP061764
• Organism: Escherichia coli O19:H7 strain 730V1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6L5EGB6
• Locus tag: ID627_RS03620
• Protein ID: WP_032669201.1
• Coordinates: 748257..748634 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A6L5EE19
• Locus tag: ID627_RS03615
• Protein ID: WP_023567998.1
• Coordinates: 747847..748206 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID627_RS03570	743108..743821	+	714	WP_032669194.1	WYL domain-containing protein	-
ID627_RS03575	743818..744453	+	636	WP_020837117.1	hypothetical protein	-
ID627_RS03580	744492..744947	+	456	WP_000754598.1	hypothetical protein	-
ID627_RS03585	744944..745393	+	450	WP_032669197.1	hypothetical protein	-
ID627_RS03590	745465..745696	+	232	Protein_711	DUF905 domain-containing protein	-
ID627_RS03595	745795..746616	+	822	WP_032669198.1	DUF945 domain-containing protein	-
ID627_RS03600	746647..747090	+	444	WP_032669199.1	antirestriction protein	-
ID627_RS03605	747102..747581	+	480	WP_032669200.1	DNA repair protein RadC	-
ID627_RS03610	747602..747823	+	222	WP_023567997.1	DUF987 domain-containing protein	-
ID627_RS03615	747847..748206	+	360	WP_023567998.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ID627_RS03620	748257..748634	+	378	WP_032669201.1	TA system toxin CbtA family protein	Toxin
ID627_RS03625	748631..749122	+	492	WP_032672023.1	hypothetical protein	-
ID627_RS03630	749152..749355	+	204	WP_032669203.1	DUF957 domain-containing protein	-
ID627_RS03635	749419..750267	+	849	WP_032669204.1	DUF4942 domain-containing protein	-
ID627_RS03645	750570..751076	+	507	WP_000228930.1	G/U mismatch-specific DNA glycosylase	-
ID627_RS03650	751155..752996	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14157.93 Da
• Isoelectric Point: 6.2236

>T172953 WP_032669201.1 NZ_CP061764:748257-748634 [Escherichia coli O19:H7]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T172953 NZ_CP080057:2050-2328 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGACGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTGTAT
ATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13192.04 Da
• Isoelectric Point: 6.3122

>AT172953 WP_023567998.1 NZ_CP061764:747847-748206 [Escherichia coli O19:H7]
MSNEIPTVNHDITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 360 bp

>AT172953 NZ_CP080057:1781-2050 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCAGAACTGAAGGATGCTTTTATGGCCGCAGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCTCATGACATGGTCGAATCCTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A6L5EGB6

Antitoxin

• AlphaFold DB: A0A6L5EE19