Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 289250..289881 Replicon chromosome
Accession NZ_CP061764
Organism Escherichia coli O19:H7 strain 730V1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ID627_RS01295 Protein ID WP_001259385.1
Coordinates 289250..289525 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ID627_RS01300 Protein ID WP_000593555.1
Coordinates 289522..289881 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID627_RS01280 284254..285321 + 1068 WP_000361470.1 HlyD family secretion protein -
ID627_RS01285 285318..288053 + 2736 WP_001547864.1 ribosome-associated ATPase/putative transporter RbbA -
ID627_RS01290 288053..289177 + 1125 WP_001488398.1 ABC transporter permease -
ID627_RS01295 289250..289525 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ID627_RS01300 289522..289881 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ID627_RS01305 290001..290402 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
ID627_RS01310 290408..291214 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
ID627_RS01315 291211..291975 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
ID627_RS01320 291975..292808 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
ID627_RS01325 292805..293749 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 281243..289881 8638


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T172951 WP_001259385.1 NZ_CP061764:289250-289525 [Escherichia coli O19:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T172951 NZ_CP080056:c4316158-4315985 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT172951 WP_000593555.1 NZ_CP061764:289522-289881 [Escherichia coli O19:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT172951 NZ_CP080056:c4316482-4316186 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References