Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5210..5735 Replicon plasmid unnamed3
Accession NZ_CP061760
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IEC98_RS24095 Protein ID WP_001159871.1
Coordinates 5430..5735 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag IEC98_RS24090 Protein ID WP_000813639.1
Coordinates 5210..5428 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS24055 1166..1383 + 218 Protein_1 hypothetical protein -
IEC98_RS24060 1387..1497 + 111 Protein_2 IS481 family transposase -
IEC98_RS24065 1769..2500 + 732 WP_000850414.1 complement resistance protein TraT -
IEC98_RS24070 2560..2931 + 372 WP_052892432.1 colicin -
IEC98_RS24075 3414..3761 + 348 WP_000142427.1 hypothetical protein -
IEC98_RS24080 3779..4369 - 591 WP_052892431.1 hypothetical protein -
IEC98_RS24085 4366..4614 - 249 WP_052892430.1 hypothetical protein -
IEC98_RS24090 5210..5428 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IEC98_RS24095 5430..5735 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IEC98_RS24100 5736..6542 + 807 WP_000016966.1 site-specific integrase -
IEC98_RS24105 6722..7366 + 645 WP_001144036.1 ParA family protein -
IEC98_RS24110 7453..7761 + 309 WP_000030199.1 hypothetical protein -
IEC98_RS24115 8176..9156 + 981 WP_000688508.1 plasmid segregation protein ParM -
IEC98_RS24120 9149..9565 + 417 WP_001278809.1 plasmid partitioning/stability family protein -
IEC98_RS24125 9567..9740 - 174 Protein_15 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..172576 172576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T172950 WP_001159871.1 NZ_CP061760:5430-5735 [Escherichia coli O68:H12]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T172950 NZ_CP080056:c4219839-4219489 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT172950 WP_000813639.1 NZ_CP061760:5210-5428 [Escherichia coli O68:H12]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT172950 NZ_CP080056:c4220084-4219833 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References