Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 146753..147489 Replicon plasmid unnamed1
Accession NZ_CP061759
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag IEC98_RS24020 Protein ID WP_003026803.1
Coordinates 146753..147235 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag IEC98_RS24025 Protein ID WP_003026799.1
Coordinates 147223..147489 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS23990 142087..142437 - 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -
IEC98_RS23995 142434..142874 - 441 WP_023307217.1 IS66 family insertion sequence hypothetical protein -
IEC98_RS24000 143030..143440 + 411 WP_053504377.1 hypothetical protein -
IEC98_RS24005 143728..144300 + 573 WP_032676095.1 recombinase family protein -
IEC98_RS24010 144309..145127 + 819 WP_023307220.1 abortive infection family protein -
IEC98_RS24015 145198..146541 - 1344 WP_080339306.1 ISNCY family transposase -
IEC98_RS24020 146753..147235 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
IEC98_RS24025 147223..147489 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
IEC98_RS24030 147934..148164 - 231 WP_023307222.1 hypothetical protein -
IEC98_RS24035 148178..148381 - 204 WP_004213596.1 hemolysin expression modulator Hha -
IEC98_RS24040 148533..149501 + 969 WP_023307223.1 IS5-like element IS903B family transposase -
IEC98_RS24045 149865..150605 + 741 WP_001515717.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..151454 151454
- inside IScluster/Tn - - 139276..149501 10225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T172949 WP_003026803.1 NZ_CP061759:c147235-146753 [Escherichia coli O68:H12]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T172949 NZ_CP080056:c4121826-4121539 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT172949 WP_003026799.1 NZ_CP061759:c147489-147223 [Escherichia coli O68:H12]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT172949 NZ_CP080056:c4121552-4121250 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References