Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4312990..4313487 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IEC98_RS20865 Protein ID WP_001173343.1
Coordinates 4312990..4313163 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IEC98_RS20870 Protein ID WP_000398619.1
Coordinates 4313191..4313487 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS20845 4308461..4309918 + 1458 WP_191115558.1 dipeptide/tripeptide permease DtpC -
IEC98_RS20850 4310155..4311495 + 1341 Protein_4096 lysine--tRNA ligase -
IEC98_RS20855 4311641..4312621 - 981 WP_032430896.1 IS5-like element ISKpn26 family transposase -
IEC98_RS20860 4312692..4312871 + 180 Protein_4098 lysine--tRNA ligase -
IEC98_RS20865 4312990..4313163 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IEC98_RS20870 4313191..4313487 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IEC98_RS20875 4313714..4313986 - 273 WP_000405647.1 N-acetyltransferase -
IEC98_RS20880 4313998..4314228 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IEC98_RS20885 4314409..4316040 + 1632 WP_001565376.1 sensor histidine kinase -
IEC98_RS20890 4316037..4316756 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4289492..4313487 23995
- flank IS/Tn - - 4311641..4312621 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T172948 WP_001173343.1 NZ_CP061758:c4313163-4312990 [Escherichia coli O68:H12]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T172948 NZ_CP080056:c3625897-3625520 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT172948 WP_000398619.1 NZ_CP061758:c4313487-4313191 [Escherichia coli O68:H12]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT172948 NZ_CP080056:c3626354-3625986 [Escherichia coli]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References