Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3555113..3555731 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IEC98_RS17290 Protein ID WP_001291435.1
Coordinates 3555513..3555731 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IEC98_RS17285 Protein ID WP_000344800.1
Coordinates 3555113..3555487 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS17275 3550202..3551395 + 1194 WP_186186822.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IEC98_RS17280 3551418..3554567 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IEC98_RS17285 3555113..3555487 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IEC98_RS17290 3555513..3555731 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IEC98_RS17295 3555903..3556454 + 552 WP_000102564.1 maltose O-acetyltransferase -
IEC98_RS17300 3556570..3557040 + 471 WP_000136192.1 YlaC family protein -
IEC98_RS17305 3557204..3558754 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IEC98_RS17310 3558796..3559149 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IEC98_RS17315 3559478..3559630 + 153 WP_162521167.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172945 WP_001291435.1 NZ_CP061758:3555513-3555731 [Escherichia coli O68:H12]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T172945 NZ_CP080056:2683996-2684103 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT172945 WP_000344800.1 NZ_CP061758:3555113-3555487 [Escherichia coli O68:H12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT172945 NZ_CP080056:c2683946-2683883 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References