Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2495195..2495833 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IEC98_RS12080 Protein ID WP_000813794.1
Coordinates 2495657..2495833 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IEC98_RS12075 Protein ID WP_001270286.1
Coordinates 2495195..2495611 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS12055 2490348..2491289 - 942 WP_001251304.1 ABC transporter permease -
IEC98_RS12060 2491290..2492302 - 1013 Protein_2373 ABC transporter ATP-binding protein -
IEC98_RS12065 2492320..2493465 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
IEC98_RS12070 2493710..2495116 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
IEC98_RS12075 2495195..2495611 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IEC98_RS12080 2495657..2495833 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IEC98_RS12085 2496055..2496285 + 231 WP_000494244.1 YncJ family protein -
IEC98_RS12090 2496377..2498338 - 1962 WP_191115495.1 23S rRNA 5-hydroxycytidine C2501 synthase -
IEC98_RS12095 2498411..2498947 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
IEC98_RS12100 2499000..2500214 + 1215 WP_071607158.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T172944 WP_000813794.1 NZ_CP061758:c2495833-2495657 [Escherichia coli O68:H12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T172944 NZ_CP080056:2683461-2683568 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT172944 WP_001270286.1 NZ_CP061758:c2495611-2495195 [Escherichia coli O68:H12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT172944 NZ_CP080056:c2683408-2683347 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References