Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1036505..1037088 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IEC98_RS04905 Protein ID WP_000254738.1
Coordinates 1036753..1037088 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IEC98_RS04900 Protein ID WP_000581937.1
Coordinates 1036505..1036753 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS04890 1032845..1034146 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IEC98_RS04895 1034194..1036428 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IEC98_RS04900 1036505..1036753 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IEC98_RS04905 1036753..1037088 + 336 WP_000254738.1 endoribonuclease MazF Toxin
IEC98_RS04910 1037159..1037950 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IEC98_RS04915 1038178..1039815 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IEC98_RS04920 1039903..1041201 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T172933 WP_000254738.1 NZ_CP061758:1036753-1037088 [Escherichia coli O68:H12]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T172933 NZ_CP080056:207393-207500 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT172933 WP_000581937.1 NZ_CP061758:1036505-1036753 [Escherichia coli O68:H12]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT172933 NZ_CP080056:c207344-207281 [Escherichia coli]
GTCTAGATGCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References