Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 913353..914007 Replicon chromosome
Accession NZ_CP061758
Organism Escherichia coli O68:H12 strain 97.3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IEC98_RS04385 Protein ID WP_000244777.1
Coordinates 913600..914007 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IEC98_RS04380 Protein ID WP_000354046.1
Coordinates 913353..913619 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC98_RS04355 908522..909265 + 744 WP_000951962.1 SDR family oxidoreductase -
IEC98_RS04360 909322..910755 - 1434 WP_191115604.1 6-phospho-beta-glucosidase BglA -
IEC98_RS04365 910800..911111 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IEC98_RS04370 911275..911934 + 660 WP_000250274.1 hemolysin III family protein -
IEC98_RS04375 912130..913110 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IEC98_RS04380 913353..913619 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IEC98_RS04385 913600..914007 + 408 WP_000244777.1 toxin CptA Toxin
IEC98_RS04390 914047..914568 - 522 WP_001055874.1 flavodoxin FldB -
IEC98_RS04395 914680..915576 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IEC98_RS04400 915601..916311 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IEC98_RS04405 916317..918050 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T172932 WP_000244777.1 NZ_CP061758:913600-914007 [Escherichia coli O68:H12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172932 NZ_CP080056:206910-207017 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172932 WP_000354046.1 NZ_CP061758:913353-913619 [Escherichia coli O68:H12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172932 NZ_CP080056:c206853-206798 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References