Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 36024..36822 Replicon plasmid unnamed3
Accession NZ_CP061757
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name ataT Uniprot ID A0A0K4UCL9
Locus tag IEC99_RS25610 Protein ID WP_024173399.1
Coordinates 36301..36822 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag IEC99_RS25605 Protein ID WP_001351987.1
Coordinates 36024..36293 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS25580 31560..32900 - 1341 WP_000137333.1 AAA family ATPase -
IEC99_RS25585 32944..33684 - 741 WP_191111805.1 hypothetical protein -
IEC99_RS25590 33878..34633 - 756 WP_032252490.1 DUF2971 domain-containing protein -
IEC99_RS25595 34678..35031 - 354 WP_164715282.1 hypothetical protein -
IEC99_RS25600 35037..35705 - 669 WP_000161228.1 AAA family ATPase -
IEC99_RS25605 36024..36293 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
IEC99_RS25610 36301..36822 + 522 WP_024173399.1 GNAT family N-acetyltransferase Toxin
IEC99_RS25615 36990..37241 - 252 WP_001404395.1 hypothetical protein -
IEC99_RS25620 37243..37935 - 693 WP_000856757.1 hypothetical protein -
IEC99_RS25625 37949..38272 - 324 WP_000064175.1 hypothetical protein -
IEC99_RS25630 38595..39203 - 609 WP_000274392.1 hypothetical protein -
IEC99_RS25635 39203..39457 - 255 WP_000120169.1 hypothetical protein -
IEC99_RS25640 39620..40147 - 528 WP_024189778.1 tail fiber assembly protein -
IEC99_RS25645 40151..41290 - 1140 WP_191111808.1 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..112745 112745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19475.31 Da        Isoelectric Point: 8.6344

>T172928 WP_024173399.1 NZ_CP061757:36301-36822 [Escherichia coli O18ac:H14]
VDNIKIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEGRPKVLGYYTLSGSCFEKESLPSRSQQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLDFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T172928 NZ_CP080029:3215191-3215451 [Streptomyces anulatus]
GTGAAGTTCGTGTGGGACCAGTCGTCCTGGGACGACTACGTCTGGTGGCGGAACCAGGATCGCAAGATCCTCAAGCGGAT
CAACACGCTGCTCCAGGACATCGCCCGGAACGGCAACGAGGGGATGGGCAAGCCGGAACCTCTGAAGCACGGCTTCCAGG
GGTACTGGTCGCGCCGCATCACCGACGAGCACCGGCTCATCTACAAGGTGGTCGACGACGAGGTCCGCGTCGCCGCCTGC
CGGTACCACTACGGCCGCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT172928 WP_001351987.1 NZ_CP061757:36024-36293 [Escherichia coli O18ac:H14]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT172928 NZ_CP080029:3214943-3215194 [Streptomyces anulatus]
ATGCGGACCATGACCTACACCGAGTCCCGCGCCAAGTACGCCGAGACCCTGAGCGCGGTCGTGGACGACCGCGAGGAGGT
CGTGGTCACGCGTGCCGGGCATGAGCCCGTGGTGATAGTGGCGCTCGACGAGTACGAGTCCCTCAAGGAGACCGCGTACC
AGCTCCGGAGCCCGGAGAACGCCCGCCGTCTGCTGGCTTCGATCGATCGCCTGGAGAACGGCGGCGGCACGGTGCGGGAG
CTCGCGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UCL9

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References