Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4139667..4140243 Replicon chromosome
Accession NZ_CP061754
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IEC99_RS20160 Protein ID WP_001297643.1
Coordinates 4139956..4140243 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag IEC99_RS20155 Protein ID WP_000063156.1
Coordinates 4139667..4139969 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS20140 4136263..4138413 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
IEC99_RS20145 4138463..4138666 + 204 WP_000467859.1 YbdD/YjiX family protein -
IEC99_RS20150 4138677..4139633 + 957 WP_001297640.1 GTPase -
IEC99_RS20155 4139667..4139969 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
IEC99_RS20160 4139956..4140243 - 288 WP_001297643.1 BrnT family toxin Toxin
IEC99_RS20165 4140442..4141139 - 698 Protein_3968 IS1 family transposase -
IEC99_RS20170 4141196..4141291 + 96 Protein_3969 dienelactone hydrolase family protein -
IEC99_RS20175 4141437..4142669 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
IEC99_RS20180 4142710..4143990 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T172926 WP_001297643.1 NZ_CP061754:c4140243-4139956 [Escherichia coli O18ac:H14]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T172926 NZ_CP080028:2595750-2596100 [Heyndrickxia oleronia]
TTGATTGTTAAGCGTGGTGACGTGTATTTTGCTGACCTTTCCCCAGTTGTCGGATCTGAACAAGGTGGAGTCCGCCCTGT
TTTGGTCATTCAAAACGACATTGGGAATCGGTTTAGTCCCACTGTAATTGTTGCTGCTATTACAGCGCAAATCCAAAAAG
CAAAACTTCCTACACATGTTGAAATTGATGCAAAGCGTTATGGCTTTGAACGCGATTCTGTTATTTTGCTTGAACAAATT
CGCACCATTGATAAACAACGCTTAACAGACAAAATCACACATTTAGACGAAGAAATGATGGAAAAAGTGGACGATGCACT
GCAAATTAGTTTAGGACTTATTGAATTTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT172926 WP_000063156.1 NZ_CP061754:c4139969-4139667 [Escherichia coli O18ac:H14]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT172926 NZ_CP080028:2595464-2595745 [Heyndrickxia oleronia]
GTGTCTGAATCCAGCGCAACAACAGAAATATTAATTCGGTTACCAGTACATTTATTATCAGAACTTGATGGATTTGCTGA
GCAAGAAAATGTGAATCGAAATGAATTTATTTATCGAGCTACAAAGATGTATCTTCGTGAGCGTAAAAAAAGACAGATTC
GCGAATCTATGAGACGCGGCTACATGGAAATGGCTAAAATTAATTTAGCAATAGCATCTGAGGCGATTCAAGCAGAGTAC
GAAGCAGAACATACAGTAGAACGCTTAGTAAGCGGGGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References