Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3616173..3616791 Replicon chromosome
Accession NZ_CP061754
Organism Escherichia coli O18ac:H14 strain 873.10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IEC99_RS17605 Protein ID WP_001291435.1
Coordinates 3616573..3616791 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IEC99_RS17600 Protein ID WP_000344800.1
Coordinates 3616173..3616547 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC99_RS17590 3611262..3612455 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IEC99_RS17595 3612478..3615627 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IEC99_RS17600 3616173..3616547 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IEC99_RS17605 3616573..3616791 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IEC99_RS17610 3616963..3617514 + 552 WP_000102564.1 maltose O-acetyltransferase -
IEC99_RS17615 3617630..3618100 + 471 WP_000136192.1 YlaC family protein -
IEC99_RS17620 3618264..3619556 + 1293 Protein_3471 cyclic-guanylate-specific phosphodiesterase PdeB -
IEC99_RS17625 3619572..3620269 - 698 Protein_3472 IS1 family transposase -
IEC99_RS17630 3620327..3620590 + 264 Protein_3473 EAL domain-containing protein -
IEC99_RS17635 3620632..3620985 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IEC99_RS17645 3621364..3621675 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3619572..3619949 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172923 WP_001291435.1 NZ_CP061754:3616573-3616791 [Escherichia coli O18ac:H14]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T172923 NZ_CP080013:1023410-1023670 [Macrococcus caseolyticus]
ATGTACAAAGTGGTTATGAAATCTAGTGTCAAGAAAGATCTGAAAAAGATAAAAAACACACACTTAGAAACGAACTTTCT
AAAAATCATTAAACAGCTTAAAGAAAATCCATATAATAATAACCATTCTTTCGAAAAACTAATTCCCCCAATAAAGGGGT
TTTATTCGAGAAGGTTAAATGTACAACATCGTGTTGTTTATACAGTAAATGACGAAGATAAAATTGTAACAATCTATTCA
GCATATGGGCGTTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT172923 WP_000344800.1 NZ_CP061754:3616173-3616547 [Escherichia coli O18ac:H14]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT172923 NZ_CP080013:1023159-1023410 [Macrococcus caseolyticus]
ATGAATACTTATACACCTACTAGTGCTAGAAAAGATTTTTTCAACATTATAAAATCTGTTAATGAAGATAGTAAGGAAAT
TTACATTGCACCAACTAAAATTGGAGAAAAAGGAGCTGTTTTAGTTGGTGAAGATGATTGGAATGCCATTCAGGAGACGT
TATTCTTATTAGAAAAAGGTATTGCTTATCAAATCGAACAACGTAAAGATGAAGAAGAAATTGATTTTGACAGTACATGG
GATAATATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References