Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 64746..65305 Replicon plasmid unnamed1
Accession NZ_CP061750
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name relE Uniprot ID U9YB49
Locus tag IED00_RS24625 Protein ID WP_000421259.1
Coordinates 65030..65305 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2A3UPL5
Locus tag IED00_RS24620 Protein ID WP_044864180.1
Coordinates 64746..65030 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS24605 60057..61536 - 1480 Protein_68 ATP-binding protein -
IED00_RS24610 61529..63589 - 2061 WP_013984538.1 DDE-type integrase/transposase/recombinase -
IED00_RS24615 63603..64430 - 828 WP_044864179.1 heteromeric transposase endonuclease subunit TnsA -
IED00_RS24620 64746..65030 + 285 WP_044864180.1 ribbon-helix-helix domain-containing protein Antitoxin
IED00_RS24625 65030..65305 + 276 WP_000421259.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IED00_RS24630 65403..65684 + 282 WP_044864186.1 hypothetical protein -
IED00_RS24635 65866..68997 + 3132 WP_044864181.1 hypothetical protein -
IED00_RS24640 69521..69934 + 414 WP_000465034.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkB / mrkC / mrkD / mrkF 1..129153 129153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10736.45 Da        Isoelectric Point: 9.9324

>T172901 WP_000421259.1 NZ_CP061750:65030-65305 [Escherichia coli O15:H12]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T172901 NZ_CP079993:3594622-3595695 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10560.00 Da        Isoelectric Point: 5.6558

>AT172901 WP_044864180.1 NZ_CP061750:64746-65030 [Escherichia coli O15:H12]
MQMKNNTAQTTKVITAHVPLPMADKIDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLNTDNPLPVPR

Download         Length: 285 bp

>AT172901 NZ_CP079993:3595688-3596059 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YB49

Antitoxin

Source ID Structure
AlphaFold DB A0A2A3UPL5

References