Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 27940..28462 Replicon plasmid unnamed1
Accession NZ_CP061750
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IED00_RS24435 Protein ID WP_000220560.1
Coordinates 28181..28462 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IED00_RS24430 Protein ID WP_000121743.1
Coordinates 27940..28191 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS24400 23325..23555 + 231 WP_000051064.1 partitioning protein -
IED00_RS24405 23571..23864 + 294 WP_015060510.1 hypothetical protein -
IED00_RS24410 23866..24834 + 969 WP_176705453.1 IS5 family transposase -
IED00_RS24415 24894..25316 - 423 WP_031942371.1 DNA distortion polypeptide 3 -
IED00_RS24420 25384..25830 - 447 WP_001074386.1 hypothetical protein -
IED00_RS24425 25870..26706 - 837 WP_001050931.1 replication initiation protein -
IED00_RS24430 27940..28191 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IED00_RS24435 28181..28462 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IED00_RS24440 28608..28931 + 324 WP_001181903.1 hypothetical protein -
IED00_RS24445 28976..29221 + 246 WP_000356546.1 hypothetical protein -
IED00_RS24450 29211..29426 + 216 WP_001180116.1 hypothetical protein -
IED00_RS24455 29519..29848 + 330 WP_000866648.1 hypothetical protein -
IED00_RS24460 29891..30070 + 180 WP_000439078.1 hypothetical protein -
IED00_RS24465 30140..30289 + 150 WP_000003880.1 hypothetical protein -
IED00_RS24470 30402..33410 - 3009 WP_001143750.1 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkB / mrkC / mrkD / mrkF 1..129153 129153


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T172900 WP_000220560.1 NZ_CP061750:28181-28462 [Escherichia coli O15:H12]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T172900 NZ_CP079993:c2944012-2943761 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT172900 WP_000121743.1 NZ_CP061750:27940-28191 [Escherichia coli O15:H12]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT172900 NZ_CP079993:c2943764-2943510 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References