Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4233906..4234456 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag IED00_RS20495 Protein ID WP_001160971.1
Coordinates 4233906..4234220 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag IED00_RS20500 Protein ID WP_000125566.1
Coordinates 4234223..4234456 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS20470 4229581..4230867 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
IED00_RS20475 4230867..4231856 + 990 WP_191111644.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IED00_RS20480 4231853..4232674 + 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IED00_RS20485 4232677..4233054 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IED00_RS20490 4233061..4233909 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IED00_RS20495 4233906..4234220 - 315 WP_001160971.1 CcdB family protein Toxin
IED00_RS20500 4234223..4234456 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IED00_RS20505 4234542..4235021 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
IED00_RS20510 4235213..4237075 - 1863 WP_000377188.1 glutathione-regulated potassium-efflux system protein KefC -
IED00_RS20515 4237068..4237598 - 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IED00_RS20520 4237709..4239040 - 1332 WP_001183198.1 MFS transporter -
IED00_RS20525 4239098..4239385 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T172894 WP_001160971.1 NZ_CP061749:c4234220-4233906 [Escherichia coli O15:H12]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T172894 NZ_CP079993:2363914-2364090 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT172894 WP_000125566.1 NZ_CP061749:c4234456-4234223 [Escherichia coli O15:H12]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT172894 NZ_CP079993:2364136-2364552 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References