172892

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3946371..3947050 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag IED00_RS19080 Protein ID WP_000854680.1
Coordinates 3946709..3947050 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag IED00_RS19075 Protein ID WP_000070396.1
Coordinates 3946371..3946688 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS19030 3941809..3942630 + 822 WP_000197388.1 DUF945 domain-containing protein -
IED00_RS19035 3942847..3943548 + 702 WP_000189411.1 WYL domain-containing protein -
IED00_RS19040 3943589..3943825 + 237 WP_001144031.1 hypothetical protein -
IED00_RS19045 3943825..3944268 + 444 WP_001547764.1 phage transcriptional regulator -
IED00_RS19050 3944291..3944758 + 468 WP_001547765.1 hypothetical protein -
IED00_RS19055 3944835..3945074 + 240 WP_000194654.1 DUF905 domain-containing protein -
IED00_RS19060 3945172..3945630 + 459 WP_001548150.1 antirestriction protein -
IED00_RS19065 3945646..3946122 + 477 WP_000811693.1 RadC family protein -
IED00_RS19070 3946131..3946352 + 222 WP_000691994.1 DUF987 domain-containing protein -
IED00_RS19075 3946371..3946688 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
IED00_RS19080 3946709..3947050 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
IED00_RS19085 3947649..3948008 + 360 WP_097311094.1 hypothetical protein -
IED00_RS19090 3948001..3948543 - 543 WP_000284450.1 plasmid pRiA4b ORF-3 family protein -
IED00_RS19095 3949147..3949428 - 282 WP_000617442.1 PerC family transcriptional regulator -
IED00_RS19100 3949688..3950143 + 456 WP_000230716.1 hypothetical protein -
IED00_RS19105 3950223..3950444 + 222 WP_001378274.1 hypothetical protein -
IED00_RS19110 3950670..3950930 - 261 WP_052601878.1 hypothetical protein -
IED00_RS19115 3950996..3951694 - 699 WP_000594596.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3941809..4003433 61624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T172892 WP_000854680.1 NZ_CP061749:3946709-3947050 [Escherichia coli O15:H12]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T172892 NZ_CP079993:c2148696-2148589 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT172892 WP_000070396.1 NZ_CP061749:3946371-3946688 [Escherichia coli O15:H12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT172892 NZ_CP079993:2148743-2148809 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References