Detailed information of TA system TA172890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3013600..3014243
• Replicon: chromosome
• Accession: NZ_CP061749
• Organism: Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: IED00_RS14565
• Protein ID: WP_088765932.1
• Coordinates: 3013600..3014016 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: IED00_RS14570
• Protein ID: WP_001261282.1
• Coordinates: 3014013..3014243 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IED00_RS14540	3009013..3009465	+	453	Protein_2872	IS110 family transposase	-
IED00_RS14545	3009747..3010928	+	1182	WP_094310466.1	DUF4268 domain-containing protein	-
IED00_RS14550	3011363..3012494	-	1132	Protein_2874	IS66 family transposase	-
IED00_RS14555	3012559..3013263	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IED00_RS14560	3013311..3013502	+	192	WP_001446477.1	helix-turn-helix domain-containing protein	-
IED00_RS14565	3013600..3014016	-	417	WP_088765932.1	type II toxin-antitoxin system VapC family toxin	Toxin
IED00_RS14570	3014013..3014243	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IED00_RS14575	3014200..3014661	+	462	WP_162542143.1	hypothetical protein	-
IED00_RS14580	3014805..3015155	+	351	WP_000493399.1	hypothetical protein	-
IED00_RS14585	3015315..3016112	-	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-
IED00_RS14590	3016112..3017284	-	1173	WP_042111630.1	IS21-like element IS21 family transposase	-
IED00_RS14595	3017370..3017549	+	180	Protein_2883	hypothetical protein	-
IED00_RS14600	3017601..3018062	-	462	Protein_2884	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3005081..3026228	21147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15164.56 Da
• Isoelectric Point: 6.4581

>T172890 WP_088765932.1 NZ_CP061749:c3014016-3013600 [Escherichia coli O15:H12]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T172890 NZ_CP079993:c1371545-1371327 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT172890 WP_001261282.1 NZ_CP061749:c3014243-3014013 [Escherichia coli O15:H12]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT172890 NZ_CP079993:c1371945-1371571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3