Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 328503..329134 Replicon chromosome
Accession NZ_CP061749
Organism Escherichia coli O15:H12 strain 2273-PO3

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag IED00_RS01490 Protein ID WP_001259385.1
Coordinates 328503..328778 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IED00_RS01495 Protein ID WP_000593555.1
Coordinates 328775..329134 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IED00_RS01475 323507..324574 + 1068 WP_000361470.1 HlyD family secretion protein -
IED00_RS01480 324571..327306 + 2736 WP_000149123.1 ribosome-associated ATPase/putative transporter RbbA -
IED00_RS01485 327306..328430 + 1125 WP_001488398.1 ABC transporter permease -
IED00_RS01490 328503..328778 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
IED00_RS01495 328775..329134 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IED00_RS01500 329254..329655 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
IED00_RS01505 329661..330467 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IED00_RS01510 330464..331228 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IED00_RS01515 331228..332061 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IED00_RS01520 332058..333002 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T172865 WP_001259385.1 NZ_CP061749:328503-328778 [Escherichia coli O15:H12]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T172865 NZ_CP079992:c2148162-2148055 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT172865 WP_000593555.1 NZ_CP061749:328775-329134 [Escherichia coli O15:H12]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT172865 NZ_CP079992:2148210-2148276 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References