Detailed information of TA system TA172864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 268310..268836
• Replicon: plasmid pECL-90-2
• Accession: NZ_CP061746
• Organism: Enterobacter hormaechei strain NJGLYY90-CR

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IFJ76_RS24015
• Protein ID: WP_000323025.1
• Coordinates: 268310..268597 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IFJ76_RS24020
• Protein ID: WP_000534858.1
• Coordinates: 268597..268836 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ76_RS23980	263880..264056	-	177	WP_001371930.1	hypothetical protein	-
IFJ76_RS23985	264567..265511	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
IFJ76_RS23990	265607..266209	+	603	WP_012695474.1	hypothetical protein	-
IFJ76_RS23995	266269..266619	+	351	WP_000743059.1	hypothetical protein	-
IFJ76_RS24000	266666..266869	+	204	WP_001015183.1	hypothetical protein	-
IFJ76_RS24005	267151..267471	+	321	WP_000332796.1	hypothetical protein	-
IFJ76_RS24010	268080..268238	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
IFJ76_RS24015	268310..268597	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IFJ76_RS24020	268597..268836	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IFJ76_RS24025	269099..270022	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
IFJ76_RS24030	270222..270794	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
IFJ76_RS24035	271270..272508	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / dfrA19 / blaTEM-1B / aac(3)-IId / blaSFO-1 / mph(A) / mcr-9 / aph(3')-Ia / tet(D) / blaSHV-12 / catA2 / sul1 / qacE / ere(A) / aac(6')-IIc / blaIMP-4	-	1..348891	348891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T172864 WP_000323025.1 NZ_CP061746:c268597-268310 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T172864 NZ_CP079992:c1371545-1371327 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT172864 WP_000534858.1 NZ_CP061746:c268836-268597 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT172864 NZ_CP079992:c1371945-1371571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8