Detailed information of TA system TA172862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37128..37925
• Replicon: plasmid pECL-90-1
• Accession: NZ_CP061745
• Organism: Enterobacter hormaechei strain NJGLYY90-CR

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: IFJ76_RS22185
• Protein ID: WP_172754447.1
• Coordinates: 37422..37925 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: IFJ76_RS22180
• Protein ID: WP_016582626.1
• Coordinates: 37128..37397 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IFJ76_RS22155	32458..33798	-	1341	WP_006812582.1	AAA family ATPase	-
IFJ76_RS22160	33859..34584	-	726	WP_058652463.1	hypothetical protein	-
IFJ76_RS22165	34848..35645	+	798	WP_077990606.1	DUF2971 domain-containing protein	-
IFJ76_RS22170	35687..36040	-	354	WP_160956455.1	hypothetical protein	-
IFJ76_RS22175	36046..36711	-	666	WP_023315962.1	AAA family ATPase	-
IFJ76_RS22180	37128..37397	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
IFJ76_RS22185	37422..37925	+	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
IFJ76_RS22190	37953..38159	-	207	WP_080399007.1	hypothetical protein	-
IFJ76_RS22195	38110..38361	-	252	WP_000147960.1	hypothetical protein	-
IFJ76_RS22200	38363..39055	-	693	WP_006812500.1	membrane protein	-
IFJ76_RS22205	39069..39392	-	324	WP_000064173.1	hypothetical protein	-
IFJ76_RS22210	39467..40255	-	789	WP_040110304.1	receptor-recognizing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106755	106755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T172862 WP_172754447.1 NZ_CP061745:37422-37925 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T172862 NZ_CP079992:1144314-1144712 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT172862 WP_016582626.1 NZ_CP061745:37128-37397 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT172862 NZ_CP079992:1144018-1144311 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3