Detailed information of TA system TA172846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5117173..5117689
• Replicon: chromosome
• Accession: NZ_CP061723
• Organism: Pseudomonas putida strain ZXPA-20

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8I0ZDP4
• Locus tag: ID616_RS23315
• Protein ID: WP_023047836.1
• Coordinates: 5117173..5117460 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1L7NIA2
• Locus tag: ID616_RS23320
• Protein ID: WP_023047837.1
• Coordinates: 5117450..5117689 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID616_RS23300	5113748..5114998	+	1251	WP_016488688.1	ribonucleotide-diphosphate reductase subunit beta	-
ID616_RS23305	5115066..5115974	+	909	WP_191086890.1	RES domain-containing protein	-
ID616_RS23310	5116335..5116871	+	537	WP_191086891.1	Bro-N domain-containing protein	-
ID616_RS23315	5117173..5117460	-	288	WP_023047836.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ID616_RS23320	5117450..5117689	-	240	WP_023047837.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ID616_RS23325	5118409..5119647	+	1239	WP_191086892.1	OprD family porin	-
ID616_RS23330	5119887..5120693	+	807	WP_023047838.1	NAD(P)-dependent oxidoreductase	-
ID616_RS23335	5120719..5121600	+	882	WP_016488690.1	SMP-30/gluconolactonase/LRE family protein	-
ID616_RS23340	5121665..5122636	+	972	WP_016488691.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11242.07 Da
• Isoelectric Point: 10.1560

>T172846 WP_023047836.1 NZ_CP061723:c5117460-5117173 [Pseudomonas putida]
MTYSLDFDARALKEWKKLGDTVRQQFKKKLAEVLLNPRIEANRLHSLPDCYKIKLRSSGFRLVYQVIDQEVVVFVVAVDR
RERDQAYKKAAERLE

Nucleotide

• Length: 288 bp

>T172846 NZ_CP079991:2931867-2932241 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9085.43 Da
• Isoelectric Point: 4.6310

>AT172846 WP_023047837.1 NZ_CP061723:c5117689-5117450 [Pseudomonas putida]
MASINIRVDDDLKARAYKELERLGVTPSELMRQALQYVAERGKLPFRPVLMTEEDEDLIATVQERLASAQRVRVQLDDL

Nucleotide

• Length: 240 bp

>AT172846 NZ_CP079991:2931410-2931778 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1L7NIA2