Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 25758..26359 Replicon plasmid pHN9-2
Accession NZ_CP061717
Organism Pediococcus acidilactici strain HN9

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag ID874_RS10295 Protein ID WP_001748110.1
Coordinates 25758..26102 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag ID874_RS10300 Protein ID WP_001748109.1
Coordinates 26096..26359 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID874_RS10260 21364..21645 + 282 WP_008855846.1 thioredoxin family protein -
ID874_RS10265 21659..21973 + 315 WP_015474701.1 thioredoxin -
ID874_RS10270 21994..22317 + 324 WP_193223782.1 thioredoxin family protein -
ID874_RS10275 22317..22451 + 135 WP_080280589.1 aspartate/glutamate racemase family protein -
ID874_RS10280 22536..23480 + 945 WP_006844822.1 thioredoxin-disulfide reductase -
ID874_RS10285 23499..24146 + 648 WP_006844821.1 DsbA family oxidoreductase -
ID874_RS10290 24308..25639 + 1332 WP_193223783.1 NAD(P)/FAD-dependent oxidoreductase -
ID874_RS10295 25758..26102 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ID874_RS10300 26096..26359 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ID874_RS10305 26445..27032 + 588 WP_050340451.1 site-specific integrase -
ID874_RS10310 27388..27600 + 213 WP_193223787.1 hypothetical protein -
ID874_RS10315 28317..29252 + 936 WP_193223784.1 replication initiation protein -
ID874_RS10320 29245..29790 + 546 WP_193223785.1 DUF536 domain-containing protein -
ID874_RS10325 30032..30565 + 534 WP_119463181.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..30711 30711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T172839 WP_001748110.1 NZ_CP061717:c26102-25758 [Pediococcus acidilactici]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T172839 NZ_CP079991:c2148161-2148054 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT172839 WP_001748109.1 NZ_CP061717:c26359-26096 [Pediococcus acidilactici]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT172839 NZ_CP079991:2148209-2148275 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References