Detailed information of TA system TA172838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 11187..11788
• Replicon: plasmid pHN9-2
• Accession: NZ_CP061717
• Organism: Pediococcus acidilactici strain HN9

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: ID874_RS10200
• Protein ID: WP_001748110.1
• Coordinates: 11187..11531 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: ID874_RS10205
• Protein ID: WP_001748109.1
• Coordinates: 11525..11788 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID874_RS10165	6793..7074	+	282	WP_008855846.1	thioredoxin family protein	-
ID874_RS10170	7088..7402	+	315	WP_015474701.1	thioredoxin	-
ID874_RS10175	7423..7746	+	324	WP_193223782.1	thioredoxin family protein	-
ID874_RS10180	7746..7880	+	135	WP_080280589.1	aspartate/glutamate racemase family protein	-
ID874_RS10185	7965..8909	+	945	WP_006844822.1	thioredoxin-disulfide reductase	-
ID874_RS10190	8928..9575	+	648	WP_006844821.1	DsbA family oxidoreductase	-
ID874_RS10195	9737..11068	+	1332	WP_193223783.1	NAD(P)/FAD-dependent oxidoreductase	-
ID874_RS10200	11187..11531	-	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ID874_RS10205	11525..11788	-	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ID874_RS10210	11874..12461	+	588	WP_050340451.1	site-specific integrase	-
ID874_RS10215	12817..13029	+	213	WP_193223787.1	hypothetical protein	-
ID874_RS10220	13746..14679	+	934	Protein_17	replication initiation protein	-
ID874_RS10225	14672..15187	+	516	WP_193223786.1	DUF536 domain-containing protein	-
ID874_RS10230	15456..15934	+	479	Protein_19	helix-turn-helix domain-containing protein	-
ID874_RS10235	15911..16309	-	399	Protein_20	ROK family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30711	30711

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T172838 WP_001748110.1 NZ_CP061717:c11531-11187 [Pediococcus acidilactici]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T172838 NZ_CP079991:c1371545-1371327 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT172838 WP_001748109.1 NZ_CP061717:c11788-11525 [Pediococcus acidilactici]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT172838 NZ_CP079991:c1371945-1371571 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A806J926