Detailed information of TA system TA172827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 2339520..2341261
• Replicon: chromosome
• Accession: NZ_CP061705
• Organism: Acinetobacter baumannii strain E-072658

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: N9S537
• Locus tag: H2787_RS11935
• Protein ID: WP_000233088.1
• Coordinates: 2340605..2341261 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: A0A427X3T1
• Locus tag: H2787_RS11930
• Protein ID: WP_002010576.1
• Coordinates: 2339520..2340605 (-)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2787_RS11910 (H2787_11910)	2335435..2336367	+	933	WP_001043692.1	IS5-like element ISAba13 family transposase	-
H2787_RS11915 (H2787_11915)	2336630..2336989	+	360	WP_000416092.1	hypothetical protein	-
H2787_RS11920 (H2787_11920)	2337083..2337388	+	306	Protein_2337	IS3 family transposase	-
H2787_RS11925 (H2787_11925)	2337855..2338730	-	876	WP_000732876.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
H2787_RS11930 (H2787_11930)	2339520..2340605	-	1086	WP_002010576.1	macro domain-containing protein	Antitoxin
H2787_RS11935 (H2787_11935)	2340605..2341261	-	657	WP_000233088.1	DUF4433 domain-containing protein	Toxin
H2787_RS11940 (H2787_11940)	2341584..2342279	-	696	Protein_2341	DUF559 domain-containing protein	-
H2787_RS11950 (H2787_11950)	2342542..2343573	-	1032	Protein_2342	FAD-dependent oxidoreductase	-
H2787_RS11955 (H2787_11955)	2343583..2344464	-	882	WP_004282326.1	IS982-like element ISAba9 family transposase	-
H2787_RS11960 (H2787_11960)	2344549..2345553	-	1005	Protein_2344	NADPH-dependent 2,4-dienoyl-CoA reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2299163..2342225	43062
	-	flank	IS/Tn	-	-	2343583..2344464	881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (18-218)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25316.92 Da
• Isoelectric Point: 7.1033

>T172827 WP_000233088.1 NZ_CP061705:c2341261-2340605 [Acinetobacter baumannii]
MVGRNYSDVLNPQKALIWRIVHRDNIPWILENGLHSSNSPTQCVNWVQIGSPELIEKRQLHPVPVGKKGVLSDYIPFYFT
PFSPMLLNIKSGRGGVRQRTNDEIVILVSSLYQLQQQQIPFVFTNSHAYYQWAEFYTDLSSLNEIDWSILQARDFKRDMD
DPAKFERYQAEALVHQHCPTKVLIGVICYTETVKAQIEQWLQQADVNIPVHVRQGVYF

Nucleotide

• Length: 657 bp

>T172827 NZ_CP079984:1253517-1253777 [Macrococcus bohemicus]
ATGTACAAAATTGTAATGAAATCTAGTGTTAAGAAAGATTTAAAGAAGATAAAAAACACGCACTTAGAAACGAACTTTCT
AAAAATCATTAAACAGCTTAAAGAAAATCCATATAATAATAACCACTCATTCGAAAAACTAATCCCCCCAATAAAGGGAT
TTTATTCGAGAAGGATAAACGTACAACATCGTGTTGTTTATACTGTAAATGACGAAGATAAAATTGCAACAATCTATTCA
GCATATGGACATTATGAGTGA

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 41042.14 Da
• Isoelectric Point: 6.0689

>AT172827 WP_002010576.1 NZ_CP061705:c2340605-2339520 [Acinetobacter baumannii]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPKNMAAYSQACKAGQVTTGKMFVTETNELMGPRWIINFPTK
QHWRDKSKLQWIEDGLQDLKKFIIDHQVKSIAIPPLGAGNGGLEWSRVKSKIETELSDLEYVDILIFEPTTQYQNVAKKI
GVSQLTPARAMIAELVRRYWVLGIECSLLEIQKLAWFLQRVIEQKQLPNELKLNFQANYYGPYASNLDHLLNAMDGSYLK
SDKRIPDCDPLDVIWFNDQQKSKVQGYLISEAKEYMAAVEGAATLIEGFESPFGMELLATVDWLIYKEGYQPNVESIQEG
LANWSAGERWARRKSDLFKAEHIQFALQKLDGFAYSHTDVN

Nucleotide

• Length: 1086 bp

>AT172827 NZ_CP079984:1253266-1253517 [Macrococcus bohemicus]
ATGAATACTTATACACCAACAAGTGCTAGAAAAGATTTTTTCAACATCATCAAATCTGTTAATGAAGATAGTAAAGAAAT
ATACATCGCACCTACTAAAATTGGAGAGAAAGGTGCTGTTTTAGTCGGTGAAGATGATTGGAATGCCATTCAGGAAACTT
TATTCTTATTAGAAAAAGGTATCGCTTATCAAATTGAACAACGTAAAGACGAAGAAGAAATTGATTTTGACAGTACTTGG
GATAATATATAA

Structures

Toxin

• AlphaFold DB: N9S537

Antitoxin

• AlphaFold DB: A0A427X3T1