Detailed information of TA system TA172824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1225688..1226605
• Replicon: chromosome
• Accession: NZ_CP061704
• Organism: Bacillus velezensis strain Y4_39

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: ID168_RS06405
• Protein ID: WP_191073306.1
• Coordinates: 1225859..1226605 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: ID168_RS06400
• Protein ID: WP_003154807.1
• Coordinates: 1225688..1225858 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ID168_RS06365	1220921..1222543	+	1623	WP_094247021.1	pyocin knob domain-containing protein	-
ID168_RS06370	1222556..1222927	+	372	WP_070081681.1	hypothetical protein	-
ID168_RS06375	1222932..1223129	+	198	WP_071391956.1	XkdX family protein	-
ID168_RS06380	1223186..1223947	+	762	WP_094247020.1	phage portal protein	-
ID168_RS06385	1223999..1224262	+	264	WP_003154815.1	hemolysin XhlA family protein	-
ID168_RS06390	1224276..1224539	+	264	WP_003154813.1	phage holin	-
ID168_RS06395	1224553..1225431	+	879	WP_045207177.1	N-acetylmuramoyl-L-alanine amidase	-
ID168_RS06400	1225688..1225858	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
ID168_RS06405	1225859..1226605	-	747	WP_191073306.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
ID168_RS06410	1226710..1227708	-	999	WP_003154805.1	inorganic phosphate transporter	-
ID168_RS06415	1227721..1228338	-	618	WP_003154804.1	DUF47 domain-containing protein	-
ID168_RS06420	1228624..1229940	-	1317	WP_094247018.1	amino acid permease	-
ID168_RS06425	1230263..1231213	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29047.54 Da
• Isoelectric Point: 4.7755

>T172824 WP_191073306.1 NZ_CP061704:c1226605-1225859 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T172824 NZ_CP079981:c1280964-1280692 [Macrococcus bohemicus]
ATGCCTAAGAAAAAGATACTATTTAATTCAAGTGGTTTTGAAGATTATACGTACTGGCAAACTAAAGATAAAACTGTATC
AAAGAAAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGTAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCCATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT172824 WP_003154807.1 NZ_CP061704:c1225858-1225688 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT172824 NZ_CP079981:c1281214-1280957 [Macrococcus bohemicus]
ATGAAAGTAACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATTATAGATATTGTTAATGAAGACTGTGAACCTTA
CACTATCACGACAAACACGCATAATGCAGTGATATTATCTGAGCATGACTATAATAGTATGATTGAAACACTTTACTTAC
AAAGTACACCTGCGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14