Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 481480..482117 Replicon chromosome
Accession NZ_CP061704
Organism Bacillus velezensis strain Y4_39

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ID168_RS02465 Protein ID WP_003156187.1
Coordinates 481767..482117 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag ID168_RS02460 Protein ID WP_003156188.1
Coordinates 481480..481761 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ID168_RS02440 477845..478444 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
ID168_RS02445 478537..478902 + 366 WP_070081400.1 holo-ACP synthase -
ID168_RS02450 479067..480074 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
ID168_RS02455 480191..481360 + 1170 WP_077721837.1 alanine racemase -
ID168_RS02460 481480..481761 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ID168_RS02465 481767..482117 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ID168_RS02470 482235..483056 + 822 WP_003156182.1 STAS domain-containing protein -
ID168_RS02475 483061..483426 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
ID168_RS02480 483429..483830 + 402 WP_003156178.1 anti-sigma regulatory factor -
ID168_RS02485 483842..484849 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
ID168_RS02490 484913..485242 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
ID168_RS02495 485239..485721 + 483 WP_007609591.1 anti-sigma B factor RsbW -
ID168_RS02500 485687..486475 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
ID168_RS02505 486475..487077 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T172823 WP_003156187.1 NZ_CP061704:481767-482117 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T172823 NZ_CP079981:1276950-1277210 [Macrococcus bohemicus]
ATGTACAAAATTGTAATGAAATCTAGTGTTAAGAAAGATTTAAAGAAGATAAAAAACACGCACTTAGAAACGAACTTTCT
AAAAATCATTAAACAGCTTAAAGAAAATCCATATAATAATAACCATTCTTTCGAAAAAATAATCCCCCCAATAAAGGGAT
TTTATTCGAGAAGGATAAACGTACAACATCGTGTTGTTTATACTGTAAATGACGAAGATAAAATTGCAACAATCTATTCA
GCATATGGACATTATGAGTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT172823 WP_003156188.1 NZ_CP061704:481480-481761 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT172823 NZ_CP079981:1276699-1276950 [Macrococcus bohemicus]
ATGAATACTTATACACCAACAAGTGCTAGAAAAGATTTTTTCAACATCATCAAATCTGTTAATGAAGATAGTAAAGAAAT
ATACATCGCACCTACTAAAATTGGAGAGAAAGGTGCTGTTTTAGTCGGTGAAGATGATTGGAATGCCATTCAGGAAACTT
TATTCTTACTAGAAAAAGGTATCGCTTATCAAATTGAACAACGTAAAGACGAAGAAGAAATTGATTTTGACAGTACTTGG
GATAATATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References