Detailed information of TA system TA172819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 194551..195308
• Replicon: plasmid pC39-334kb
• Accession: NZ_CP061701
• Organism: Klebsiella pneumoniae strain C39

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: IE968_RS26630
• Protein ID: WP_029497408.1
• Coordinates: 194551..195033 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: IE968_RS26635
• Protein ID: WP_094645258.1
• Coordinates: 195021..195308 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE968_RS26605	190233..190610	+	378	WP_042927811.1	hypothetical protein	-
IE968_RS26610	190973..191944	-	972	WP_009653249.1	hypothetical protein	-
IE968_RS26615	192484..192744	+	261	WP_009653224.1	hypothetical protein	-
IE968_RS26620	192952..193113	-	162	WP_017900895.1	type I toxin-antitoxin system Hok family toxin	-
IE968_RS26625	193183..194262	+	1080	WP_009653230.1	IS481 family transposase	-
IE968_RS26630	194551..195033	-	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
IE968_RS26635	195021..195308	-	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
IE968_RS26640	195545..196468	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
IE968_RS26645	196668..197240	-	573	WP_094645259.1	cytochrome b/b6 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / sul1 / blaVEB-3 / mph(A) / blaSFO-1 / aac(3)-IId / blaTEM-1B / ARR-3 / dfrA27 / qacE / qnrA7	katA	1..334893	334893
	-	inside	IScluster/Tn	-	-	193183..198569	5386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T172819 WP_029497408.1 NZ_CP061701:c195033-194551 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T172819 NZ_CP079955:c1027425-1027153 [Macrococcus sp. 19Msa1099]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAAACTAAAGATAAAACTGTATC
AAAGAAAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGTAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCCATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT172819 WP_094645258.1 NZ_CP061701:c195308-195021 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT172819 NZ_CP079955:c1027675-1027418 [Macrococcus sp. 19Msa1099]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGCATAATGCAGTGATATTATCTGAGCATGACTATAATAGTATGATTGAAACACTTTACTTAC
AAAGTACACCTGCGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7