Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5212097..5212722 Replicon chromosome
Accession NZ_CP061700
Organism Klebsiella pneumoniae strain C39

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag IE968_RS25250 Protein ID WP_004187928.1
Coordinates 5212097..5212480 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IE968_RS25255 Protein ID WP_004150355.1
Coordinates 5212480..5212722 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE968_RS25235 5209463..5210365 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IE968_RS25240 5210362..5210997 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IE968_RS25245 5210994..5211923 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IE968_RS25250 5212097..5212480 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
IE968_RS25255 5212480..5212722 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IE968_RS25260 5212916..5213833 + 918 WP_004187929.1 alpha/beta hydrolase -
IE968_RS25265 5213847..5214788 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IE968_RS25270 5214833..5215270 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IE968_RS25275 5215267..5216127 - 861 WP_004146232.1 virulence factor BrkB family protein -
IE968_RS25280 5216121..5216720 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T172818 WP_004187928.1 NZ_CP061700:c5212480-5212097 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172818 NZ_CP079955:1023411-1023671 [Macrococcus sp. 19Msa1099]
ATGTACAAAGTGGTTATGAAATCTAGTGTCAAGAAAGATCTGAAAAAGATAAAAAACACACACTTAGAAACGAACTTTCT
AAAAATCATTAAACAGCTTAAAGAAAATCCATATAATAATAACCATTCTTTCGAAAAACTAATTCCCCCAATAAAGGGGT
TTTATTCGAGAAGGTTAAATGTACAACATCGTGTTGTTTATACAGTAAATGACGAAGATAAAATTGTAACAATCTATTCA
GCATATGGGCGTTATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172818 WP_004150355.1 NZ_CP061700:c5212722-5212480 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172818 NZ_CP079955:1023160-1023411 [Macrococcus sp. 19Msa1099]
ATGAATACTTATACACCTACTAGTGCTAGAAAAGATTTTTTCAACATTATAAAATCTGTTAATGAAGATAGTAAGGAAAT
TTACATTGCACCAACTAAAATTGGAGAAAAAGGAGCTGTTTTAGTTGGTGAAGATGATTGGAATGCCATTCAGGAGACGT
TATTCTTATTAGAAAAAGGTATTGCTTATCAAATCGAACAACGTAAAGATGAAGAAGAAATTGATTTTGACAGTACATGG
GATAATATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References