172774

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 2735..3323 Replicon plasmid pAS39-3
Accession NZ_CP061649
Organism Acinetobacter seifertii strain AS39

Toxin (Protein)


Gene name brnT Uniprot ID A0A7H2VD82
Locus tag IC776_RS19110 Protein ID WP_023271598.1
Coordinates 2735..3022 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9PH66
Locus tag IC776_RS19115 Protein ID WP_005098515.1
Coordinates 3009..3323 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC776_RS19095 46..594 + 549 WP_006582660.1 plasmid replication DNA-binding protein -
IC776_RS19100 1123..1266 - 144 Protein_1 transposase -
IC776_RS19105 1525..2475 + 951 WP_006582641.1 GGDEF domain-containing protein -
IC776_RS19110 2735..3022 + 288 WP_023271598.1 BrnT family toxin Toxin
IC776_RS19115 3009..3323 + 315 WP_005098515.1 BrnA antitoxin family protein Antitoxin
IC776_RS19120 3400..4725 - 1326 WP_016805832.1 ISNCY family transposase -
IC776_RS19125 4894..5229 + 336 WP_025466893.1 hypothetical protein -
IC776_RS19130 5279..5458 + 180 WP_001231652.1 hypothetical protein -
IC776_RS19135 5455..5841 + 387 WP_000053135.1 type II toxin-antitoxin system death-on-curing family toxin -
IC776_RS19140 5843..6394 - 552 WP_000755487.1 macro domain-containing protein -
IC776_RS19145 6403..7101 - 699 WP_006582673.1 hypothetical protein -
IC776_RS19150 7409..7654 - 246 WP_006582672.1 helix-turn-helix domain-containing protein -
IC776_RS19155 7807..8049 + 243 WP_006582671.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11979 11979
- inside IScluster/Tn - - 1108..4737 3629


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11291.66 Da        Isoelectric Point: 5.2216

>T172774 WP_023271598.1 NZ_CP061649:2735-3022 [Acinetobacter seifertii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Download         Length: 288 bp

>T172774 NZ_CP079924:1790454-1790708 [Escherichia sp. TC-EC600-tetX4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12270.93 Da        Isoelectric Point: 8.4842

>AT172774 WP_005098515.1 NZ_CP061649:3009-3323 [Acinetobacter seifertii]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT172774 NZ_CP079924:1790206-1790457 [Escherichia sp. TC-EC600-tetX4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H2VD82

Antitoxin

Source ID Structure
AlphaFold DB N9PH66

References