Detailed information of TA system TA172762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-AbkA/BrnT-BrnA
• Location: 2286..2874
• Replicon: plasmid pAS48-2
• Accession: NZ_CP061628
• Organism: Acinetobacter seifertii strain AS48

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: IC781_RS18995
• Protein ID: WP_151676414.1
• Coordinates: 2286..2573 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: AbkA
• Uniprot ID: -
• Locus tag: IC781_RS19000
• Protein ID: WP_151676413.1
• Coordinates: 2560..2874 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC781_RS18990	288..1775	-	1488	WP_000953323.1	SulP family inorganic anion transporter	-
IC781_RS18995	2286..2573	+	288	WP_151676414.1	BrnT family toxin	Toxin
IC781_RS19000	2560..2874	+	315	WP_151676413.1	BrnA antitoxin family protein	Antitoxin
IC781_RS19005	3176..4831	+	1656	WP_191011571.1	histone H1-like repetitive region-containing protein	-
IC781_RS19010	4921..6321	+	1401	WP_151676417.1	DUF560 domain-containing protein	-
IC781_RS19015	6605..6829	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
IC781_RS19020	6979..7224	+	246	WP_004282220.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12619	12619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11285.67 Da
• Isoelectric Point: 5.2232

>T172762 WP_151676414.1 NZ_CP061628:2286-2573 [Acinetobacter seifertii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGKVRGVLMMLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYENG

Nucleotide

• Length: 288 bp

>T172762 NZ_CP079923:c1098416-1098162 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAACCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTCGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12288.83 Da
• Isoelectric Point: 7.3183

>AT172762 WP_151676413.1 NZ_CP061628:2560-2874 [Acinetobacter seifertii]
MRTVRYSRKELNEKFSDKQDAEIERLLAKGTGPDDHLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILREAMLEDLKNHR

Nucleotide

• Length: 315 bp

>AT172762 NZ_CP079923:c1098660-1098418 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA