Detailed information of TA system TA172750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1855024..1856660
• Replicon: chromosome
• Accession: NZ_CP061596
• Organism: Acinetobacter seifertii strain AS59

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: IC788_RS08740
• Protein ID: WP_191001613.1
• Coordinates: 1855024..1856355 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: N8S5T2
• Locus tag: IC788_RS08745
• Protein ID: WP_004701524.1
• Coordinates: 1856352..1856660 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC788_RS08720	1850024..1851610	+	1587	WP_191001610.1	GH3 auxin-responsive promoter family protein	-
IC788_RS08725	1851697..1852935	+	1239	WP_191001698.1	TolC family protein	-
IC788_RS08730	1852928..1854091	+	1164	WP_191001611.1	hypothetical protein	-
IC788_RS08735	1854291..1854731	-	441	WP_191001612.1	HD domain-containing protein	-
IC788_RS08740	1855024..1856355	+	1332	WP_191001613.1	HipA domain-containing protein	Toxin
IC788_RS08745	1856352..1856660	+	309	WP_004701524.1	helix-turn-helix domain-containing protein	Antitoxin
IC788_RS08750	1857389..1857970	+	582	WP_191001614.1	TetR/AcrR family transcriptional regulator	-
IC788_RS08755	1858088..1858927	+	840	WP_191001615.1	2OG-Fe dioxygenase family protein	-
IC788_RS08760	1858924..1859817	+	894	WP_191001616.1	lysine exporter LysO family protein	-
IC788_RS08765	1859935..1860453	+	519	WP_191001617.1	GNAT family N-acetyltransferase	-
IC788_RS08770	1860516..1861439	-	924	WP_191001699.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (167-365)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-83)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51079.90 Da
• Isoelectric Point: 4.9719

>T172750 WP_191001613.1 NZ_CP061596:1855024-1856355 [Acinetobacter seifertii]
MKKLTIQALLNKNWIDIAELKLLEPKLGSASASELEYELDYAIQYSDRRDEHACSLSLPVQILIKHESNTWFGFLDDIVP
SGAARRYWVNYLDLQRLTHAEQESILLEKGGMAPVGNLRIKEALPPLNPESTLHLRYFSKEDVAERNIDFLEYAQQMGAI
SGGATGAGGEAPKLLIRVSPEQQVWIDTYQQQFDQPDQHYLVKFPRNKRSEIDCDILRAEYYYYQILNELGFNTIETTQM
QLIEGTKYPSLWLPRFDTEWRDQSWHRHGLESVYSILNKSSGSHLNHFEVIEELCRLLTSIDSDFDASQFVCEWLQRDLL
NIIFGNSDNHGRNTSFLKKSGKIYLSPIYDFAPMKADPEVVTRSTTWGSPYEEGGEYRWAQITQKLATWCDPAVSLATLK
TLANNLLGLRQRLVDKDVPKQIIEMPALSFDYIEAKLQRWELL

Nucleotide

• Length: 1332 bp

>T172750 NZ_CP079903:4707151-4707333 [Pseudomonas juntendi]
GTGCAAAGCAGGCAGTTGATAAAGGAGCTTGAAGCAGCTGGATGGGTTCTAAAACGAGTCACCGGAAGCCACCACATCTT
CAAACACCCCAACAACCCAAACTCGATACCAGTGCCACACCCCAAGAAGGACCTGCCGATCGGCACGGTAAAGAGCATCA
AGGAACGAGCCGGGTTGAAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11590.52 Da
• Isoelectric Point: 9.9701

>AT172750 WP_004701524.1 NZ_CP061596:1856352-1856660 [Acinetobacter seifertii]
MNIKNQTQDRQQVLIDLYKQYLLSEITLGQLLSYLRKNVLGLSQEQYATLVGISRRTLTDIEQDKGKLTQSVLDKVFKPL
GLKAGLVPTHEHIVSKIIKPSE

Nucleotide

• Length: 309 bp

>AT172750 NZ_CP079903:4707371-4707772 [Pseudomonas juntendi]
ATGCAATACCCAATCTGCATCGAGTGGGGCGACGAGAACACCGCCACCGGCATTCAGATCCCCGATATTCCAGGCGCTGT
TACCGCGGGCGACACCTTCGAGGAGGCCTATTCCGCAGCTGTCGAGGTAGCCCACATCATGCTGGAAGAGATCGCCAGTA
ACGGCCAGGCCATCCCGATGCCAACCAGCGCCGCGGCCCACCGGGACAATCCCGACTTCGCTGACATGGGCTGGGGCATG
CTGGAGATCGACATCACGCCATACCTGGGCAAGACCGAAAAGGTCAATGTCACCCTGCCGGGCTTCGTCATCCAGCAGAT
TGACCGGTACGTACGCGATCACAATGTGAAGAGCCGCTCATCGTTCTTGGCCGATGCAGCCATGGAAAAGCTGGGCCGCT
GA

Structures

Antitoxin

• AlphaFold DB: N8S5T2