Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 5817..6401 Replicon plasmid pAS73-2
Accession NZ_CP061563
Organism Acinetobacter seifertii strain AS73

Toxin (Protein)


Gene name PumA Uniprot ID A0A7H2PX46
Locus tag IC796_RS20220 Protein ID WP_049066985.1
Coordinates 5817..6119 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7H2PX47
Locus tag IC796_RS20225 Protein ID WP_049066984.1
Coordinates 6120..6401 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC796_RS20190 1352..1705 + 354 WP_191036796.1 hypothetical protein -
IC796_RS20195 1945..2148 - 204 WP_151685843.1 hypothetical protein -
IC796_RS20200 2198..2389 - 192 WP_151685845.1 hypothetical protein -
IC796_RS20205 3507..4430 + 924 WP_151685847.1 replication initiation protein RepM -
IC796_RS20210 4497..5033 + 537 WP_151685849.1 plasmid replication DNA-binding protein -
IC796_RS20215 5244..5672 - 429 WP_030423737.1 hypothetical protein -
IC796_RS20220 5817..6119 + 303 WP_049066985.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IC796_RS20225 6120..6401 + 282 WP_049066984.1 putative addiction module antidote protein Antitoxin
IC796_RS20230 6599..6934 + 336 WP_004740263.1 carboxymuconolactone decarboxylase family protein -
IC796_RS20235 7221..7649 + 429 Protein_11 hypothetical protein -
IC796_RS20240 7574..7774 + 201 Protein_12 GABA permease -
IC796_RS20245 8176..10284 + 2109 WP_151685841.1 TonB-dependent siderophore receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..14469 14469


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-91)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11589.07 Da        Isoelectric Point: 4.6838

>T172742 WP_049066985.1 NZ_CP061563:5817-6119 [Acinetobacter seifertii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLSGGDKS
TQSKDIKLALQLAQDLEEEL

Download         Length: 303 bp

>T172742 NZ_CP079891:4383958-4384260 [Escherichia fergusonii]
ATGACTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10151.75 Da        Isoelectric Point: 8.0018

>AT172742 WP_049066984.1 NZ_CP061563:6120-6401 [Acinetobacter seifertii]
MAIKLRKWDSAEHLKTEEDMQAYLQVCIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Download         Length: 282 bp

>AT172742 NZ_CP079891:4384296-4384637 [Escherichia fergusonii]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H2PX46

Antitoxin

Source ID Structure
AlphaFold DB A0A7H2PX47

References