Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 32380..32902 Replicon plasmid p2
Accession NZ_CP061532
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag H5V41_RS23430 Protein ID WP_000220560.1
Coordinates 32380..32661 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag H5V41_RS23435 Protein ID WP_000121743.1
Coordinates 32651..32902 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS23385 28727..29023 - 297 WP_000916182.1 TrbC/VirB2 family protein -
H5V41_RS23390 29001..29273 - 273 WP_001436757.1 hypothetical protein -
H5V41_RS23395 29345..30322 + 978 WP_190812260.1 IS5 family transposase -
H5V41_RS23400 30552..30701 - 150 WP_000003880.1 hypothetical protein -
H5V41_RS23405 30771..30950 - 180 WP_000439078.1 hypothetical protein -
H5V41_RS23410 30993..31322 - 330 WP_000866648.1 hypothetical protein -
H5V41_RS23415 31415..31630 - 216 WP_001180116.1 hypothetical protein -
H5V41_RS23420 31620..31865 - 246 WP_000356546.1 hypothetical protein -
H5V41_RS23425 31911..32234 - 324 WP_001181903.1 hypothetical protein -
H5V41_RS23430 32380..32661 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H5V41_RS23435 32651..32902 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
H5V41_RS23440 34136..34972 + 837 WP_001050931.1 replication initiation protein -
H5V41_RS23445 35012..35458 + 447 WP_001074386.1 hypothetical protein -
H5V41_RS23450 35526..35948 + 423 WP_031942371.1 DNA distortion polypeptide 3 -
H5V41_RS23455 36008..36976 - 969 WP_176705453.1 IS5 family transposase -
H5V41_RS23460 37010..37207 + 198 Protein_42 EAL domain-containing protein -
H5V41_RS23465 37242..37876 - 635 Protein_43 type 3 fimbria minor subunit MrkF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkF / mrkD / mrkC / mrkB / mrkA / mrkJ / mrkF / mrkD / mrkD / mrkC / mrkC / mrkC / mrkB 1..85479 85479
- flank IS/Tn - - 29345..30322 977
- flank IS/Tn - - 36008..36931 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T172733 WP_000220560.1 NZ_CP061532:c32661-32380 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T172733 NZ_CP079891:c2881163-2881056 [Escherichia fergusonii]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATCGCTGGCATTCTTGCCAGTTTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT172733 WP_000121743.1 NZ_CP061532:c32902-32651 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT172733 NZ_CP079891:2881211-2881277 [Escherichia fergusonii]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References