Detailed information of TA system TA172732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 32307..33105
• Replicon: plasmid p1
• Accession: NZ_CP061531
• Organism: Escherichia coli strain WEM25

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H5V41_RS22845
• Protein ID: WP_032187665.1
• Coordinates: 32584..33105 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A141BRA2
• Locus tag: H5V41_RS22840
• Protein ID: WP_001711191.1
• Coordinates: 32307..32576 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V41_RS22815	27747..29087	-	1341	WP_000137333.1	AAA family ATPase	-
H5V41_RS22820	29131..29871	-	741	WP_072652149.1	hypothetical protein	-
H5V41_RS22825	30244..30921	+	678	WP_072652150.1	hypothetical protein	-
H5V41_RS22830	30960..31313	-	354	WP_162521425.1	hypothetical protein	-
H5V41_RS22835	31319..31987	-	669	WP_000161229.1	AAA family ATPase	-
H5V41_RS22840	32307..32576	+	270	WP_001711191.1	DUF1778 domain-containing protein	Antitoxin
H5V41_RS22845	32584..33105	+	522	WP_032187665.1	GNAT family N-acetyltransferase	Toxin
H5V41_RS22850	33274..33525	-	252	WP_077108702.1	hypothetical protein	-
H5V41_RS22855	33527..34219	-	693	WP_052988648.1	hypothetical protein	-
H5V41_RS22860	34233..34556	-	324	WP_000064175.1	hypothetical protein	-
H5V41_RS22865	34631..35413	-	783	WP_077108701.1	receptor-recognizing protein	-
H5V41_RS22870	35480..37555	-	2076	Protein_40	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108197	108197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (48-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19345.11 Da
• Isoelectric Point: 7.7881

>T172732 WP_032187665.1 NZ_CP061531:32584-33105 [Escherichia coli]
VSNTTIEIFSGENDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T172732 NZ_CP079891:c2432785-2432468 [Escherichia fergusonii]
ATGCAAATCTTATCATCACAACCGACACAGGCGGCTCAGCCCTGTCTTTCGCCCGTCGAAATCTGGCAACGGCTGCTGAC
TCATCTGTTGTTGAAGCACTATGGTCTGGCGCTGAATGACACACCATTCAGTAATGAAACCGTTATTCAGGAGCACATTG
ATGCCGGTATCACTATGGCTAACGCTATGAATTTTCTGGTAGAAAAATATGAGTTGGTTCGTATTGACCGCAGGGAATTC
GACTGGCAAGAAAAGTCCCCATACCTGCAGGCTGTCGATATTCTGCGGGCGCGACAGGCTACAGGTATGCGGCAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10054.53 Da
• Isoelectric Point: 8.7353

>AT172732 WP_001711191.1 NZ_CP061531:32307-32576 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Nucleotide

• Length: 270 bp

>AT172732 NZ_CP079891:c2433189-2432824 [Escherichia fergusonii]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTACAGAGTACGATCACACC
CTGTTTTGGAGCTCGTCTGGTGCAGGAAGGCAACCGCCTGCATTTTCTCGCTGACCGGGCCGGATTCAACGGTGCTTTCA
GTGACGATGACGCGCTACACCTAGAGCAGACCTTTCCGTTGATACTTAAGCAACTGGAGATGATGCTCACCAGCGGAGAG
CTTTCTCCCCGACATCAGCACTCCGTCACGCTGTATCACAATGGACTATCTTGCGAAGCCGATACACTCGGCTCCTGTGG
TTATATCTACATCGCTATTTATCCCGACCAGACTGAGCCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A141BRA2