Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3607152..3607806 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H5V41_RS17525 Protein ID WP_000244777.1
Coordinates 3607152..3607559 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H5V41_RS17530 Protein ID WP_000354046.1
Coordinates 3607540..3607806 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS17505 3603109..3604842 - 1734 WP_190811970.1 single-stranded-DNA-specific exonuclease RecJ -
H5V41_RS17510 3604848..3605558 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H5V41_RS17515 3605583..3606479 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H5V41_RS17520 3606591..3607112 + 522 WP_001055874.1 flavodoxin FldB -
H5V41_RS17525 3607152..3607559 - 408 WP_000244777.1 toxin CptA Toxin
H5V41_RS17530 3607540..3607806 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H5V41_RS17535 3608049..3609029 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H5V41_RS17540 3609225..3609884 - 660 WP_000250274.1 hemolysin III family protein -
H5V41_RS17545 3610048..3610359 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H5V41_RS17550 3610404..3611837 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H5V41_RS17555 3611894..3612637 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T172728 WP_000244777.1 NZ_CP061530:c3607559-3607152 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172728 NZ_CP079891:c1996163-1995765 [Escherichia fergusonii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCCAAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACA
AATGACGAAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172728 WP_000354046.1 NZ_CP061530:c3607806-3607540 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172728 NZ_CP079891:c1996459-1996166 [Escherichia fergusonii]
ATGCATCGAATTCTTGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATATTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References