Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2158463..2158989 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H5V41_RS10480 Protein ID WP_000323025.1
Coordinates 2158463..2158750 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H5V41_RS10485 Protein ID WP_000534858.1
Coordinates 2158750..2158989 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS10445 2154145..2155104 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
H5V41_RS10450 2155297..2155821 + 525 WP_000780584.1 lipocalin family protein -
H5V41_RS10455 2155977..2156354 - 378 WP_001204787.1 antitermination protein -
H5V41_RS10460 2156372..2157421 - 1050 WP_190812214.1 DUF968 domain-containing protein -
H5V41_RS10465 2157423..2157701 - 279 WP_001515373.1 hypothetical protein -
H5V41_RS10470 2157768..2158019 - 252 WP_001463433.1 hypothetical protein -
H5V41_RS10475 2158236..2158391 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H5V41_RS10480 2158463..2158750 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H5V41_RS10485 2158750..2158989 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H5V41_RS10490 2159014..2159319 + 306 WP_071593935.1 hypothetical protein -
H5V41_RS10495 2159522..2159854 + 333 WP_001301033.1 protein FlxA -
H5V41_RS10500 2160291..2161631 - 1341 WP_000589013.1 ISNCY family transposase -
H5V41_RS10505 2162170..2163332 + 1163 WP_126323058.1 IS3-like element IS3 family transposase -
H5V41_RS10510 2163526..2163774 + 249 Protein_2047 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2146263..2183282 37019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T172717 WP_000323025.1 NZ_CP061530:c2158750-2158463 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T172717 NZ_CP079884:c4451553-4451446 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT172717 WP_000534858.1 NZ_CP061530:c2158989-2158750 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT172717 NZ_CP079884:4451601-4451667 [Escherichia fergusonii]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References