Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2025516..2026154 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H5V41_RS09860 Protein ID WP_000813794.1
Coordinates 2025516..2025692 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H5V41_RS09865 Protein ID WP_001270286.1
Coordinates 2025738..2026154 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS09840 2021135..2022349 - 1215 WP_001326689.1 BenE family transporter YdcO -
H5V41_RS09845 2022402..2022938 + 537 WP_113453391.1 DNA-binding transcriptional regulator SutR -
H5V41_RS09850 2023011..2024972 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H5V41_RS09855 2025064..2025294 - 231 WP_000494244.1 YncJ family protein -
H5V41_RS09860 2025516..2025692 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H5V41_RS09865 2025738..2026154 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H5V41_RS09870 2026233..2027639 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
H5V41_RS09875 2027884..2029029 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
H5V41_RS09880 2029047..2030060 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H5V41_RS09885 2030061..2031002 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T172715 WP_000813794.1 NZ_CP061530:2025516-2025692 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T172715 NZ_CP079884:c4450105-4449998 [Escherichia fergusonii]
ATGACGCTCGCTGAGTTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT172715 WP_001270286.1 NZ_CP061530:2025738-2026154 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT172715 NZ_CP079884:4450163-4450217 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References