Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 990914..991532 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H5V41_RS04750 Protein ID WP_001291435.1
Coordinates 990914..991132 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H5V41_RS04755 Protein ID WP_000344800.1
Coordinates 991158..991532 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS04715 986203..986775 + 573 WP_190812110.1 YbaY family lipoprotein -
H5V41_RS04720 986806..987117 - 312 WP_000409911.1 MGMT family protein -
H5V41_RS04730 987496..987849 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H5V41_RS04735 987891..989441 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H5V41_RS04740 989605..990075 - 471 WP_000136192.1 YlaC family protein -
H5V41_RS04745 990191..990742 - 552 WP_000102564.1 maltose O-acetyltransferase -
H5V41_RS04750 990914..991132 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H5V41_RS04755 991158..991532 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H5V41_RS04760 992078..995227 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H5V41_RS04765 995250..996443 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172710 WP_001291435.1 NZ_CP061530:c991132-990914 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T172710 NZ_CP079884:c3557005-3556628 [Escherichia fergusonii]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT172710 WP_000344800.1 NZ_CP061530:c991532-991158 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT172710 NZ_CP079884:c3557462-3557094 [Escherichia fergusonii]
GTGTCAGACACGTTCCATGAAACAAATTATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References