Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 498987..499563 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H5V41_RS02355 Protein ID WP_001297643.1
Coordinates 498987..499274 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag H5V41_RS02360 Protein ID WP_190812071.1
Coordinates 499261..499563 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS02335 495240..496520 - 1281 WP_001037400.1 DUF445 domain-containing protein -
H5V41_RS02340 496561..497793 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
H5V41_RS02345 497939..498034 - 96 Protein_451 dienelactone hydrolase family protein -
H5V41_RS02350 498091..498788 + 698 Protein_452 IS1 family transposase -
H5V41_RS02355 498987..499274 + 288 WP_001297643.1 BrnT family toxin Toxin
H5V41_RS02360 499261..499563 + 303 WP_190812071.1 BrnA antitoxin family protein Antitoxin
H5V41_RS02365 499597..500553 - 957 WP_001297640.1 GTPase -
H5V41_RS02370 500564..500767 - 204 WP_000467859.1 YbdD/YjiX family protein -
H5V41_RS02375 500817..502967 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T172708 WP_001297643.1 NZ_CP061530:498987-499274 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T172708 NZ_CP079884:c2710683-2710528 [Escherichia fergusonii]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAATCTGAGGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11507.96 Da        Isoelectric Point: 9.4646

>AT172708 WP_190812071.1 NZ_CP061530:499261-499563 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRVDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT172708 NZ_CP079884:2710695-2710753 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure

References