Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 293716..294213 Replicon chromosome
Accession NZ_CP061530
Organism Escherichia coli strain WEM25

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H5V41_RS01335 Protein ID WP_001173343.1
Coordinates 294040..294213 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H5V41_RS01330 Protein ID WP_000398619.1
Coordinates 293716..294012 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5V41_RS01310 290446..291165 - 720 WP_000611288.1 two-component system response regulator DcuR -
H5V41_RS01315 291162..292793 - 1632 WP_001216486.1 sensor histidine kinase -
H5V41_RS01320 292974..293204 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
H5V41_RS01325 293216..293488 + 273 WP_000405647.1 N-acetyltransferase -
H5V41_RS01330 293716..294012 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H5V41_RS01335 294040..294213 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H5V41_RS01340 294337..294948 - 612 Protein_255 lysine--tRNA ligase -
H5V41_RS01345 294968..295864 - 897 Protein_256 IS3 family transposase -
H5V41_RS01350 295874..297025 - 1152 WP_001254932.1 IS30-like element IS30 family transposase -
H5V41_RS01355 297090..297338 - 249 Protein_258 transposase -
H5V41_RS01360 297404..297907 - 504 Protein_259 lysine--tRNA ligase -
H5V41_RS01365 297962..298659 + 698 Protein_260 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T172707 WP_001173343.1 NZ_CP061530:294040-294213 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T172707 NZ_CP079884:1957927-1958030 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT172707 WP_000398619.1 NZ_CP061530:293716-294012 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT172707 NZ_CP079884:c1958068-1957801 [Escherichia fergusonii]
AAAGTCAGCGAAGGGAATTTTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCGT
TCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References