Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3545172..3545722 Replicon chromosome
Accession NZ_CP061527
Organism Shigella dysenteriae strain HNCMB 20080

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag IC625_RS18885 Protein ID WP_001160974.1
Coordinates 3545172..3545486 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID D3H431
Locus tag IC625_RS18890 Protein ID WP_000125565.1
Coordinates 3545489..3545722 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC625_RS18860 3540847..3542133 + 1287 WP_000800471.1 peptidylprolyl isomerase SurA -
IC625_RS18865 3542133..3543122 + 990 WP_000241239.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IC625_RS18870 3543119..3543940 + 822 WP_001065369.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IC625_RS18875 3543943..3544320 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IC625_RS18880 3544327..3545175 + 849 WP_000257202.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IC625_RS18885 3545172..3545486 - 315 WP_001160974.1 CcdB family protein Toxin
IC625_RS18890 3545489..3545722 - 234 WP_000125565.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IC625_RS18895 3545808..3546287 - 480 WP_005020907.1 type 3 dihydrofolate reductase -
IC625_RS18900 3546479..3548146 - 1668 WP_000377138.1 glutathione-regulated potassium-efflux system protein -
IC625_RS18905 3548139..3548669 - 531 WP_000600719.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IC625_RS18910 3548780..3550111 - 1332 Protein_3692 MFS transporter -
IC625_RS18915 3550169..3550456 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T172705 WP_001160974.1 NZ_CP061527:c3545486-3545172 [Shigella dysenteriae]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T172705 NZ_CP079884:1247235-1247537 [Escherichia fergusonii]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8599.64 Da        Isoelectric Point: 6.9791

>AT172705 WP_000125565.1 NZ_CP061527:c3545722-3545489 [Shigella dysenteriae]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRRF

Download         Length: 234 bp

>AT172705 NZ_CP079884:1247573-1247914 [Escherichia fergusonii]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A090NGC7

References