Detailed information of TA system TA172702

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2658018..2658816
• Replicon: chromosome
• Accession: NZ_CP061527
• Organism: Shigella dysenteriae strain HNCMB 20080

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q32HN1
• Locus tag: IC625_RS14245
• Protein ID: WP_001094433.1
• Coordinates: 2658442..2658816 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q32HN0
• Locus tag: IC625_RS14240
• Protein ID: WP_001285603.1
• Coordinates: 2658018..2658395 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC625_RS14200	2653198..2653665	+	468	WP_000668907.1	hypothetical protein	-
IC625_RS14205	2654096..2654579	+	484	Protein_2777	hypothetical protein	-
IC625_RS14210	2654699..2654872	+	174	WP_000088742.1	DUF905 family protein	-
IC625_RS14215	2654973..2655794	+	822	WP_001234573.1	DUF945 domain-containing protein	-
IC625_RS14220	2656074..2656547	+	474	WP_001313575.1	antirestriction protein	-
IC625_RS14225	2656563..2657015	+	453	WP_001186198.1	RadC family protein	-
IC625_RS14230	2657084..2657305	+	222	WP_000692331.1	DUF987 domain-containing protein	-
IC625_RS14235	2657324..2657968	+	645	WP_000086764.1	hypothetical protein	-
IC625_RS14240	2658018..2658395	+	378	WP_001285603.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IC625_RS14245	2658442..2658816	+	375	WP_001094433.1	toxin	Toxin
IC625_RS14250	2658813..2659304	+	492	WP_000976859.1	hypothetical protein	-
IC625_RS14255	2659316..2659513	+	198	WP_000839257.1	DUF957 domain-containing protein	-
IC625_RS14260	2659610..2660452	+	843	WP_005020348.1	DUF4942 domain-containing protein	-
IC625_RS14270	2660923..2661861	+	939	WP_000351336.1	glyoxylate/hydroxypyruvate reductase GhrA	-
IC625_RS14275	2661916..2662653	+	738	WP_000283663.1	zinc-binding phosphatase	-
IC625_RS14280	2662677..2663231	+	555	WP_001001927.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13918.86 Da
• Isoelectric Point: 6.8517

>T172702 WP_001094433.1 NZ_CP061527:2658442-2658816 [Shigella dysenteriae]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSPLINSIDILRARRVTGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T172702 NZ_CP079884:718572-718886 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCATTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13789.72 Da
• Isoelectric Point: 6.4643

>AT172702 WP_001285603.1 NZ_CP061527:2658018-2658395 [Shigella dysenteriae]
VSDTLPGTTLPDDNKDRPWWRLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT172702 NZ_CP079884:718336-718569 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A090NBY3

Antitoxin

• AlphaFold DB: A0A090NW75