Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 654492..655146 Replicon chromosome
Accession NZ_CP061527
Organism Shigella dysenteriae strain HNCMB 20080

Toxin (Protein)


Gene name cptA Uniprot ID Q32BV9
Locus tag IC625_RS03430 Protein ID WP_000244779.1
Coordinates 654492..654899 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IC625_RS03435 Protein ID WP_000354046.1
Coordinates 654880..655146 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC625_RS03410 650449..652182 - 1734 WP_000813170.1 single-stranded-DNA-specific exonuclease RecJ -
IC625_RS03415 652188..652898 - 711 WP_000715209.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IC625_RS03420 652923..653819 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IC625_RS03425 653931..654452 + 522 WP_001055874.1 flavodoxin FldB -
IC625_RS03430 654492..654899 - 408 WP_000244779.1 protein YgfX Toxin
IC625_RS03435 654880..655146 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IC625_RS03440 655389..656369 + 981 WP_000886092.1 tRNA-modifying protein YgfZ -
IC625_RS03445 656446..657042 - 597 Protein_665 hemolysin III family protein -
IC625_RS03450 657100..657794 + 695 WP_087786203.1 IS1-like element IS1N family transposase -
IC625_RS03455 658043..658354 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IC625_RS03460 658399..659832 + 1434 Protein_668 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 657399..657794 395


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.2669

>T172691 WP_000244779.1 NZ_CP061527:c654899-654492 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRWILLQQETQR

Download         Length: 408 bp

>T172691 NZ_CP079870:9971-10273 [Mycobacterium sp. SMC-4]
GTGATCGCTCCCGGGGACATCGCCCCGCGTCGCGATACCAGCCATGAGGTCTATGTTGTCGTGCTATCCAACTCGATTCA
CCTGGCGGCCGATACCGGACGAGTCATCGCCTGCCCCTTTATTCCGGGCAAAATTCCCGACGCCGCAATGGCGTTCGTCG
TTCGAGTCGGACAACCCGAAGGCGTGGCGCTGCCCGAACTTGTTCAGTGGCTTCCCGCAGCAGCGCTGGACGAGCCGATC
GGCAACATCGGCGCGAATGCGCTCCGTGACACAGCGTCTCTCGTGACAGCCCTCGTCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172691 WP_000354046.1 NZ_CP061527:c655146-654880 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172691 NZ_CP079870:9747-9974 [Mycobacterium sp. SMC-4]
ATGGCCAAAGACAAGGTGTCGATCACACTGGATCAGGATGTGCTAGCAGCGGCAGATGCAGACGCCAAAGCGGCTGGGCT
CAACCGATCCGAGTTAGTTGAGCAGGCATTGCGCCACGAACACCTCCGGATCGCTCTGAAAAGCTACATGACGACAACCG
TCCCCGCCCTGGACATCGACGACTACGCACAGAAGATCTACCAGGCGAATCGGGCCTCGGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWM4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References