Detailed information of TA system TA172664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3388040..3389760
• Replicon: chromosome
• Accession: NZ_CP061498
• Organism: Roseicitreum antarcticum strain ZS2-28

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: H9529_RS16195
• Protein ID: WP_092886938.1
• Coordinates: 3389119..3389760 (-)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: H9529_RS16190
• Protein ID: WP_092886935.1
• Coordinates: 3388040..3389119 (-)
• Length: 360 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9529_RS16180	3383746..3385413	+	1668	WP_143033486.1	hypothetical protein	-
H9529_RS16185	3385940..3387958	-	2019	WP_092887132.1	ATP-binding protein	-
H9529_RS16190	3388040..3389119	-	1080	WP_092886935.1	macro domain-containing protein	Antitoxin
H9529_RS16195	3389119..3389760	-	642	WP_092886938.1	DUF4433 domain-containing protein	Toxin
H9529_RS16200	3390933..3392465	+	1533	WP_092887135.1	ABC transporter substrate-binding protein	-
H9529_RS16205	3392644..3393627	+	984	WP_092886941.1	ABC transporter permease	-
H9529_RS16210	3393624..3394568	+	945	WP_092886944.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3351535..3402601	51066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (13-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24455.95 Da
• Isoelectric Point: 7.9859

>T172664 WP_092886938.1 NZ_CP061498:c3389760-3389119 [Roseicitreum antarcticum]
MANNLTAEKALIFRITHINNVPWILRHGLHCKNSGVQDPHFVRIGNLELIQRRTARNVPVPPGGTLSDYIPFYLTPFSMM
MYNIKTGYGGIRQFPNREIVIMASSLRGLADRSVATVFSDRHAYLQTAQFFTSLDDLDKIDWGILQRRDFTRDVDDPEKT
DRYQAEALVHRHLPVEHLAGIVCLGENEKGTLERQREEVGHDLKIVARPGWYF

Nucleotide

• Length: 642 bp

>T172664 NZ_CP079855:c670526-670257 [Agrobacterium fabrum]
GTGATCTGGACAATTGAATATCACACGCTTGTGCAGAAAGAGATGCGCAAAATAAATCCGGAAGTGCGCCGGCGCATCCG
CAGTTTTCTCCATGAAAGGCTGGCCGCACTGGACGACCCGCGCCAGATCGGCGCTACACTGCAAGGTTCCGAACTCGGAA
ATTTCTGGCGTTACCGGGTGGGCGATTACCGCATCATCTGCGATATACAGGACCAAAAGCTGGTCGTTCTGGTGGTCGAA
ATCGGCCATCGCCGTGAAATTTACCGTTAG

Antitoxin

Protein

• Length: 360 a.a.
• Molecular weight: 40333.35 Da
• Isoelectric Point: 5.5671

>AT172664 WP_092886935.1 NZ_CP061498:c3389119-3388040 [Roseicitreum antarcticum]
MVTYMQGNLLEADVDAVVNTVNTVGIMGKGIALMFKEQFPRNFEAYARACDAGEVRIGKMFVTENKELFGPRWIINFPTK
THWRVKTKIEWVEDGLKDLVRVLREKEIHSIAIPPLGCGNGGLDWSDVRPLIESALAELDWVNVSVYEPTSKYQNVAKRT
GVEKLTPARALVAEMVRRYSLLGIECSILEVQKLGWFLERGMTRFGGSDALKFRFQANKYGPYSHNLTKLLDSLDGSYLR
CDRRLADADPLDLICFNDAKYDRVQAYLNSGEGKQFSSVLEWASATIDGFESPLGMELLATVDWMMQYDDIEPTVEGVME
GLKSWAGGEAAGQRKLKIFDRRLVAIALGQLQTSNRLPA

Nucleotide

• Length: 1080 bp

>AT172664 NZ_CP079855:c670737-670510 [Agrobacterium fabrum]
ATGAGCAAACAGACGGCCATTCGCCTGCCGGACGAGACCTATGAGCGGCTGAAGGCGCTGTCTGAGCGGACCGGCCGCAC
ATCCGCCTATTATATCCGCGAGGCGATCGAGAAGCATATCGAGGATATGGAAGACCTCTATCTTGCCGAAGAGGCGACGC
GGCGCATTCAGCGCGGAGAATCGAAGATTATAAGCGCCGAGGAGTTCTGGCGTGATCTGGACAATTGA