Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 75312..75889 Replicon plasmid pCRE-055-2
Accession NZ_CP061406
Organism Klebsiella pneumoniae strain NU-CRE055

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag HCV81_RS28085 Protein ID WP_059065587.1
Coordinates 75557..75889 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag HCV81_RS28080 Protein ID WP_032454911.1
Coordinates 75312..75557 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV81_RS28050 70919..71509 + 591 WP_023317848.1 hypothetical protein -
HCV81_RS28055 71538..71897 + 360 WP_032440413.1 hypothetical protein -
HCV81_RS28060 72026..72574 + 549 WP_023317849.1 phospholipase D family protein -
HCV81_RS28065 72764..73030 + 267 WP_023317850.1 replication regulatory protein RepA -
HCV81_RS28070 73253..73330 + 78 WP_020802013.1 RepA leader peptide Tap -
HCV81_RS28075 73323..74186 + 864 WP_023317851.1 incFII family plasmid replication initiator RepA -
HCV81_RS28080 75312..75557 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HCV81_RS28085 75557..75889 + 333 WP_059065587.1 endoribonuclease MazF Toxin
HCV81_RS28090 76149..76472 + 324 WP_021312689.1 hypothetical protein -
HCV81_RS28095 76655..76909 - 255 WP_004152108.1 hypothetical protein -
HCV81_RS28100 77290..77492 - 203 Protein_77 hemolysin expression modulator Hha -
HCV81_RS28105 77526..77894 - 369 WP_004152105.1 hypothetical protein -
HCV81_RS28110 77938..78428 - 491 Protein_79 DNA-binding protein -
HCV81_RS28115 78459..79034 - 576 WP_004152103.1 hypothetical protein -
HCV81_RS28120 79022..79291 - 270 WP_004152102.1 hypothetical protein -
HCV81_RS28125 79878..80822 + 945 WP_057064680.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib - 1..85405 85405


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T172648 WP_059065587.1 NZ_CP061406:75557-75889 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T172648 NZ_CP079838:190182-190334 [Salmonella enterica subsp. salamae]
ATGCCGCAGAAAGGTATGTTATACAGTTTAATAGTGATATGTCTCACTATTTTATTATTTACCTGGATAGTTCGTGATTC
ACTGTGTGAATTACATATCCGCCAGGGAAATAATGAGCTGGCGGCATCTTTAGCCTGTGATAGCAAACAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT172648 WP_032454911.1 NZ_CP061406:75312-75557 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT172648 NZ_CP079838:c190131-190076 [Salmonella enterica subsp. salamae]
GTTCAAGCATAGAGGTGGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References