Detailed information of TA system TA172647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2872..3407
• Replicon: plasmid pCRE-055-2
• Accession: NZ_CP061406
• Organism: Klebsiella pneumoniae strain NU-CRE055

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HCV81_RS27730
• Protein ID: WP_016531292.1
• Coordinates: 2872..3159 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A837JUA1
• Locus tag: HCV81_RS27735
• Protein ID: WP_023317799.1
• Coordinates: 3156..3407 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV81_RS27715	1..531	+	531	WP_059065768.1	hypothetical protein	-
HCV81_RS27720	785..1072	-	288	WP_016529486.1	hypothetical protein	-
HCV81_RS27725	1162..1824	-	663	WP_023317797.1	hypothetical protein	-
HCV81_RS27730	2872..3159	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HCV81_RS27735	3156..3407	-	252	WP_023317799.1	hypothetical protein	Antitoxin
HCV81_RS27740	3503..4771	-	1269	WP_023317800.1	Y-family DNA polymerase	-
HCV81_RS27745	4771..5202	-	432	WP_023317801.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HCV81_RS27750	5608..7095	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
HCV81_RS27755	7344..8315	+	972	WP_023317803.1	plasmid stability protein StbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-3 / aac(6')-Ib	-	1..85405	85405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T172647 WP_016531292.1 NZ_CP061406:c3159-2872 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T172647 NZ_CP079837:c4542876-4542565 [Salmonella enterica subsp. salamae]
ATGATATTTATAGAGACGGAGATTTTTACCGAAGACGTCAAAGATTTATTGGATGACGATGAGTACCACCGATTACAACT
GTTCCTTGCGGTACAACCTGAGAGCGGTGACCTTATACAGGATAGCGGCGGTTTGAGGAAGATTCGTTGGGGCGTTCGGG
GAAGGGGGAAGCGTAGCGGTGTAAGAATCATTTACTTTTATCGGGCCAGTCGATCTGAAATTCGCCTGCTGCTGATTTAT
AAAAAAGGGCTTAAAGATGATTTAACCGTACAAGAAAAGGCGGTGCTTCGTATGTTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.60 Da
• Isoelectric Point: 4.2108

>AT172647 WP_023317799.1 NZ_CP061406:c3407-3156 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT172647 NZ_CP079837:c4542564-4542274 [Salmonella enterica subsp. salamae]
ATGGATAAAGCGTTATTTGAGCGTTTAACGAAAAGCATGACTCAAATGAATGAGATCGTTGAGGGGGGGGGCCAGCCTTC
ACGCACCTTTTATATTGATGCCATGAAAATTAAAGAAATTCGACAAGCCTCTGGGTTATCACAGACAAAATTTGCGGATC
TCATTTCCGTTAGCGTAGATACGTTGCGCAATTGGGAGCAAGGCAGACGTTCACCTACCGGGCCAGCAAAAGCCTTGCTG
CGCGCGATCGCTAATGACCCTGAGCATGTGATATCCGCGTTAGGCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A837JUA1