Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 102526..103262 Replicon plasmid pCRE-055-1
Accession NZ_CP061405
Organism Klebsiella pneumoniae strain NU-CRE055

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HCV81_RS27420 Protein ID WP_003026803.1
Coordinates 102526..103008 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HCV81_RS27425 Protein ID WP_003026799.1
Coordinates 102996..103262 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV81_RS27405 98163..99125 + 963 WP_004152113.1 M48 family metalloprotease -
HCV81_RS27410 99213..100467 + 1255 Protein_105 IS3 family transposase -
HCV81_RS27415 100968..102314 - 1347 WP_020314316.1 ISNCY family transposase -
HCV81_RS27420 102526..103008 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HCV81_RS27425 102996..103262 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HCV81_RS27430 103438..103693 - 256 Protein_109 hypothetical protein -
HCV81_RS27435 103769..104026 - 258 WP_004152107.1 hypothetical protein -
HCV81_RS27440 104075..104278 - 204 WP_004152106.1 hemolysin expression modulator Hha -
HCV81_RS27445 104312..104680 - 369 WP_004152105.1 hypothetical protein -
HCV81_RS27450 104724..105218 - 495 WP_004152104.1 DNA-binding protein -
HCV81_RS27455 105249..105824 - 576 WP_004152103.1 hypothetical protein -
HCV81_RS27460 105812..106081 - 270 WP_004152102.1 hypothetical protein -
HCV81_RS27465 106439..106789 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HCV81_RS27470 106839..107201 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..162789 162789


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T172646 WP_003026803.1 NZ_CP061405:c103008-102526 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T172646 NZ_CP079837:c4243184-4242699 [Salmonella enterica subsp. salamae]
ATGATCTCCGCACCAGAACCGCTTCACGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCAATGAAGAACCAGCTTTCCGGCGCATCCCGCACGTTTGTCTGCTGCGACGATGTTAAGGTGATGG
CTTACTACTCGCTGGCTTCAAGCGCAGTGGCGACAAATACTGCGCCCGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTGCTGGGCCGTCTGGCCGTGGATCAATCGCTGCATGGGCGGGGCGTTGGACGGGCGCTGATGCGGGATGC
CGGGCTGCGGGTGATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGTTGGTTCACGCTCTGTCGGATGAAGCGCGGG
AGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTTCAT
GCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT172646 WP_003026799.1 NZ_CP061405:c103262-102996 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT172646 NZ_CP079837:c4243474-4243181 [Salmonella enterica subsp. salamae]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGCATCAAGCCCGCCGAGCGCGATCTTATCGA
TCGGGGGGCGAAAGCCAGAGGGAAAAACCGCACTGATTTTGTGCTGGAGGCCGCCCGCGCCGCCGCCGAGGAAGCGCTGA
TCGAGCAGCGCATCATTATGGCCGATCCGCAGGCTTATCAGGCGTTTCTGACCCGTCTGGATCAGGCTCCCGCGCCAAAC
GATGCATTGCGTAAAACCATGCAGACTCCCGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References